Western blot - QKI Rabbit mAb (A0193)
Western blot analysis of various lysates using QKI Rabbit mAb (A0193) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Immunohistochemistry - QKI Rabbit mAb (A0193)
Immunohistochemistry analysis of QKI in paraffin-embedded human breast cancer tissue using QKI Rabbit mAb (A0193) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry - QKI Rabbit mAb (A0193)
Immunohistochemistry analysis of QKI in paraffin-embedded Human lung adenocarcinoma tissue using QKI Rabbit mAb (A0193) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry - QKI Rabbit mAb (A0193)
Immunohistochemistry analysis of QKI in paraffin-embedded human thyroid tissue using QKI Rabbit mAb (A0193) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry - QKI Rabbit mAb (A0193)
Immunohistochemistry analysis of QKI in paraffin-embedded human tonsil tissue using QKI Rabbit mAb (A0193) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry - QKI Rabbit mAb (A0193)
Immunohistochemistry analysis of QKI in paraffin-embedded mouse kidney tissue using QKI Rabbit mAb (A0193) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry - QKI Rabbit mAb (A0193)
Immunohistochemistry analysis of QKI in paraffin-embedded mouse liver tissue using QKI Rabbit mAb (A0193) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry - QKI Rabbit mAb (A0193)
Immunohistochemistry analysis of QKI in paraffin-embedded rat kidney tissue using QKI Rabbit mAb (A0193) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunofluorescence - QKI Rabbit mAb (A0193)
Immunofluorescence analysis of NIH/3T3 cells using QKI Rabbit mAb (A0193) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunoprecipitation - QKI Rabbit mAb (A0193)
Immunoprecipitation analysis of 300 μg extracts of NIH/3T3 cells using 3 μg QKI antibody (A0193). Western blot was performed from the immunoprecipitate using QK1 antibody (A0193) at a dilution of 1:1000.