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QKI Rabbit mAb (A22723)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - QKI Rabbit mAb (A22723)

Western blot analysis of various lysates, using QKI Rabbit mAb (A22723) at 1:20000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - QKI Rabbit mAb (A22723)

Immunohistochemistry analysis of paraffin-embedded Human placenta using QKI Rabbit mAb (A22723) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - QKI Rabbit mAb (A22723)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using QKI Rabbit mAb (A22723) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - QKI Rabbit mAb (A22723)

Immunohistochemistry analysis of paraffin-embedded Rat lung using QKI Rabbit mAb (A22723) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - QKI Rabbit mAb (A22723)

Confocal imaging of NIH-3T3 cells using QKI Rabbit mAb (A22723, dilution 1:200)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 60x.

Overview

Product nameQKI Rabbit mAb
Catalog No.A22723
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC57310
The protein encoded by this gene is an RNA-binding protein that regulates pre-mRNA splicing, export of mRNAs from the nucleus, protein translation, and mRNA stability. The encoded protein is involved in myelinization and oligodendrocyte differentiation and may play a role in schizophrenia. Multiple transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-341 of human QKI. (NP_006766.1).
SequenceMVGEMETKEKPKPTPDYLMQLMNDKKLMSSLPNFCGIFNHLERLLDEEISRVRKDMYNDTLNGSTEKRSAELPDAVGPIVQLQEKLYVPVKEYPDFNFVGRILGPRGLTAKQLEAETGCKIMVRGKGSMRDKKKEEQNRGKPNWEHLNEDLHVLITVEDAQNRAEIKLKRAVEEVKKLLVPAAEGEDSLKKMQLMELAILNGTYRDANIKSPALAFSLAATAQAAPRIITGPAPVLPPAALRTPTPAGPTIMPLIRQIQTAVMPNGTPHPTAAIVPPGPEAGLIYTPYEYPYTLAPATSILEYPIEPSGVLGAVATKVRRHDMRVHPYQRIVTADRAATGN
Gene ID
Swiss Prot
SynonymsQK; Hqk; QK1; QK3; hqkI; QKI
Calculated MW38kDa
Observed MW42kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2000 - 1:20000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, K-562, Neuro-2a, NIH/3T3, C2C12
Cellular locationCytoplasm, Nucleus

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - QKI Rabbit mAb (A22723)}

    Western blot - QKI Rabbit mAb (A22723)

    Western blot analysis of various lysates, using QKI Rabbit mAb (A22723) at 1:20000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Immunohistochemistry - QKI Rabbit mAb (A22723)}

    Immunohistochemistry - QKI Rabbit mAb (A22723)

    Immunohistochemistry analysis of paraffin-embedded Human placenta using QKI Rabbit mAb (A22723) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - QKI Rabbit mAb (A22723)}

    Immunohistochemistry - QKI Rabbit mAb (A22723)

    Immunohistochemistry analysis of paraffin-embedded Mouse brain using QKI Rabbit mAb (A22723) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - QKI Rabbit mAb (A22723)}

    Immunohistochemistry - QKI Rabbit mAb (A22723)

    Immunohistochemistry analysis of paraffin-embedded Rat lung using QKI Rabbit mAb (A22723) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunofluorescence - QKI Rabbit mAb (A22723)}

    Immunofluorescence - QKI Rabbit mAb (A22723)

    Confocal imaging of NIH-3T3 cells using QKI Rabbit mAb (A22723, dilution 1:200)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 60x.

    * For research use only. Not for therapeutic or diagnostic purposes.

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    Secondary Antibodies (26)