제품 > 항체 > 다중클론항체(pAb)

RAB11A/RAB11B Rabbit pAb (A20286)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - RAB11A/RAB11B Rabbit pAb (A20286)

Western blot analysis of various lysates using RAB11A/RAB11B Rabbit pAb (A20286) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - RAB11A/RAB11B Rabbit pAb (A20286)

Western blot analysis of various lysates using RAB11A/RAB11B Rabbit pAb (A20286) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - RAB11A/RAB11B Rabbit pAb (A20286)

Immunohistochemistry analysis of paraffin-embedded Human brain using RAB11A/RAB11B Rabbit pAb (A20286) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - RAB11A/RAB11B Rabbit pAb (A20286)

Immunohistochemistry analysis of paraffin-embedded Human liver using RAB11A/RAB11B Rabbit pAb (A20286) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - RAB11A/RAB11B Rabbit pAb (A20286)

Immunohistochemistry analysis of paraffin-embedded Mouse heart using RAB11A/RAB11B Rabbit pAb (A20286) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - RAB11A/RAB11B Rabbit pAb (A20286)

Immunohistochemistry analysis of paraffin-embedded Rat brain using RAB11A/RAB11B Rabbit pAb (A20286) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - RAB11A/RAB11B Rabbit pAb (A20286)

Immunofluorescence analysis of A431 cells using RAB11A/RAB11B Rabbit pAb (A20286) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - RAB11A/RAB11B Rabbit pAb (A20286)

Immunofluorescence analysis of C6 cells using RAB11A/RAB11B Rabbit pAb (A20286) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - RAB11A/RAB11B Rabbit pAb (A20286)

Immunofluorescence analysis of HeLa cells using RAB11A/RAB11B Rabbit pAb (A20286) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - RAB11A/RAB11B Rabbit pAb (A20286)

Immunofluorescence analysis of NIH/3T3 cells using RAB11A/RAB11B Rabbit pAb (A20286) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - RAB11A/RAB11B Rabbit pAb (A20286)

Immunofluorescence analysis of U2OS cells using RAB11A/RAB11B Rabbit pAb (A20286) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameRAB11A/RAB11B Rabbit pAb
Catalog No.A20286
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. The small Rab GTPase RAB11A regulates endocytic recycling. Acts as a major regulator of membrane delivery during cytokinesis. Together with MYO5B and RAB8A participates in epithelial cell polarization. Together with RAB3IP, RAB8A, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B participates in CFTR trafficking to the plasma membrane and TF (Transferrin) recycling in nonpolarized cells. Required in a complex with MYO5B and RAB11FIP2 for the transport of NPC1L1 to the plasma membrane. Participates in the sorting and basolateral transport of CDH1 from the Golgi apparatus to the plasma membrane. Regulates the recycling of FCGRT (receptor of Fc region of monomeric Ig G) to basolateral membranes. May also play a role in melanosome transport and release from melanocytes.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human RAB11A/RAB11B (NP_004654.1).
SequenceMGTRDDEYDYLFKVVLIGDSGVGKSNLLSRFTRNEFNLESKSTIGVEFATRSIQVDGKTIKAQIWDTAGQERYRAITSAYYRGAVGALLVYDIAKHLTYE
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW25kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesA-549, Mouse brain, Mouse heart, Rat liver, Rat heart
Cellular locationaxon, centriole, centrosome, cytoplasmic vesicle, cytosol, endosome, extracellular exosome, glutamatergic synapse, Golgi apparatus, multivesicular body, postsynaptic recycling endosome, recycling endosome, spindle pole, trans-Golgi network.

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - RAB11A/RAB11B Rabbit pAb (A20286)}

    Western blot - RAB11A/RAB11B Rabbit pAb (A20286)

    Western blot analysis of various lysates using RAB11A/RAB11B Rabbit pAb (A20286) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Western blot - RAB11A/RAB11B Rabbit pAb (A20286)}

    Western blot - RAB11A/RAB11B Rabbit pAb (A20286)

    Western blot analysis of various lysates using RAB11A/RAB11B Rabbit pAb (A20286) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Immunohistochemistry - RAB11A/RAB11B Rabbit pAb (A20286)}

    Immunohistochemistry - RAB11A/RAB11B Rabbit pAb (A20286)

    Immunohistochemistry analysis of paraffin-embedded Human brain using RAB11A/RAB11B Rabbit pAb (A20286) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - RAB11A/RAB11B Rabbit pAb (A20286)}

    Immunohistochemistry - RAB11A/RAB11B Rabbit pAb (A20286)

    Immunohistochemistry analysis of paraffin-embedded Human liver using RAB11A/RAB11B Rabbit pAb (A20286) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - RAB11A/RAB11B Rabbit pAb (A20286)}

    Immunohistochemistry - RAB11A/RAB11B Rabbit pAb (A20286)

    Immunohistochemistry analysis of paraffin-embedded Mouse heart using RAB11A/RAB11B Rabbit pAb (A20286) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - RAB11A/RAB11B Rabbit pAb (A20286)}

    Immunohistochemistry - RAB11A/RAB11B Rabbit pAb (A20286)

    Immunohistochemistry analysis of paraffin-embedded Rat brain using RAB11A/RAB11B Rabbit pAb (A20286) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunofluorescence - RAB11A/RAB11B Rabbit pAb (A20286)}

    Immunofluorescence - RAB11A/RAB11B Rabbit pAb (A20286)

    Immunofluorescence analysis of A431 cells using RAB11A/RAB11B Rabbit pAb (A20286) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - RAB11A/RAB11B Rabbit pAb (A20286)}

    Immunofluorescence - RAB11A/RAB11B Rabbit pAb (A20286)

    Immunofluorescence analysis of C6 cells using RAB11A/RAB11B Rabbit pAb (A20286) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - RAB11A/RAB11B Rabbit pAb (A20286)}

    Immunofluorescence - RAB11A/RAB11B Rabbit pAb (A20286)

    Immunofluorescence analysis of HeLa cells using RAB11A/RAB11B Rabbit pAb (A20286) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - RAB11A/RAB11B Rabbit pAb (A20286)}

    Immunofluorescence - RAB11A/RAB11B Rabbit pAb (A20286)

    Immunofluorescence analysis of NIH/3T3 cells using RAB11A/RAB11B Rabbit pAb (A20286) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - RAB11A/RAB11B Rabbit pAb (A20286)}

    Immunofluorescence - RAB11A/RAB11B Rabbit pAb (A20286)

    Immunofluorescence analysis of U2OS cells using RAB11A/RAB11B Rabbit pAb (A20286) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)