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RAB1B Rabbit pAb (A21669)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - RAB1B Rabbit pAb (A21669)

Western blot analysis of various lysates using RAB1B Rabbit pAb (A21669) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - RAB1B Rabbit pAb (A21669)

Immunofluorescence analysis of C6 cells using RAB1B Rabbit pAb (A21669) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - RAB1B Rabbit pAb (A21669)

Immunofluorescence analysis of NIH-3T3 cells using RAB1B Rabbit pAb (A21669) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - RAB1B Rabbit pAb (A21669)

Immunofluorescence analysis of U-2 OS cells using RAB1B Rabbit pAb (A21669) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameRAB1B Rabbit pAb
Catalog No.A21669
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Members of the RAB protein family, such as RAB1B, are low molecular mass monomeric GTPases localized on the cytoplasmic surfaces of distinct membrane-bound organelles. RAB1B functions in the early secretory pathway and is essential for vesicle transport between the endoplasmic reticulum (ER) and Golgi (Chen et al., 1997 [PubMed 9030196]; Alvarez et al., 2003 [PubMed 12802079]).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 7-201 of human RAB1B (NP_112243.1).
SequenceYLFKLLLIGDSGVGKSCLLLRFADDTYTESYISTIGVDFKIRTIELDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIVVYDVTDQESYANVKQWLQEIDRYASENVNKLLVGNKSDLTTKKVVDNTTAKEFADSLGIPFLETSAKNATNVEQAFMTMAAEIKKRMGPGAASGGERPNLKIDSTPVKPAGGGCC
Gene ID
Swiss Prot
SynonymsRAB1B; ras-related protein Rab-1B
Calculated MW22kDa
Observed MWRefer to figures
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesNCI-H460, SK-OV-3, 293T, MCF7, Mouse kidney, Mouse heart, Mouse brain
Cellular locationCytoplasm, Cytoplasmic side, Lipid-anchor, Membrane, Preautophagosomal structure membrane

Documents

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ABclonal:Western blot - RAB1B Rabbit pAb (A21669)}

Western blot - RAB1B Rabbit pAb (A21669)

Western blot analysis of various lysates using RAB1B Rabbit pAb (A21669) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - RAB1B Rabbit pAb (A21669)}

Immunofluorescence - RAB1B Rabbit pAb (A21669)

Immunofluorescence analysis of C6 cells using RAB1B Rabbit pAb (A21669) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - RAB1B Rabbit pAb (A21669)}

Immunofluorescence - RAB1B Rabbit pAb (A21669)

Immunofluorescence analysis of NIH-3T3 cells using RAB1B Rabbit pAb (A21669) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - RAB1B Rabbit pAb (A21669)}

Immunofluorescence - RAB1B Rabbit pAb (A21669)

Immunofluorescence analysis of U-2 OS cells using RAB1B Rabbit pAb (A21669) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (1)

Secondary Antibodies (26)