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RAB5C Rabbit pAb (A7342)

Publications (3) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - RAB5C Rabbit pAb (A7342)

Western blot analysis of extracts of various cell lines, using RAB5C antibody (A7342) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.

Overview

Product nameRAB5C Rabbit pAb
Catalog No.A7342
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Members of the Rab protein family are small GTPases of the Ras superfamily that are thought to ensure fidelity in the process of docking and/or fusion of vesicles with their correct acceptor compartment (Han et al., 1996 [PubMed 8646882]).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-216 of human RAB5C (NP_958842.1).
SequenceMAGRGGAARPNGPAAGNKICQFKLVLLGESAVGKSSLVLRFVKGQFHEYQESTIGAAFLTQTVCLDDTTVKFEIWDTAGQERYHSLAPMYYRGAQAAIVVYDITNTDTFARAKNWVKELQRQASPNIVIALAGNKADLASKRAVEFQEAQAYADDNSLLFMETSAKTAMNVNEIFMAIAKKLPKNEPQNATGAPGRNRGVDLQENNPASRSQCCSN
Gene ID
Swiss Prot
SynonymsRABL; L1880; RAB5L; RAB5CL
Calculated MW23kDa
Observed MW23kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesA-549, BT-474, HL-60, Mouse lung
Cellular locationCell membrane, Cytoplasmic side, Early endosome membrane, Lipid-anchor, Melanosome
Customer validation

WB(Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

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ABclonal:Western blot - RAB5C Rabbit pAb (A7342)}

Western blot - RAB5C Rabbit pAb (A7342)

Western blot analysis of extracts of various cell lines, using RAB5C antibody (A7342) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (2)

Secondary Antibodies (26)