제품 > 항체 > 다중클론항체(pAb)

RAB9A Rabbit pAb (A7041)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of various lysates using RAB9A Rabbit pAb (A7041) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product name	RAB9A Rabbit pAb
Catalog No.	A7041
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Enables GDP binding activity; GTP binding activity; and GTPase activity. Involved in negative regulation by host of symbiont catalytic activity; positive regulation of exocytosis; and regulation of protein localization. Located in late endosome; lysosome; and phagocytic vesicle.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-201 of human RAB9A (NP_004242.1).
Sequence	MAGKSSLFKVILLGDGGVGKSSLMNRYVTNKFDTQLFHTIGVEFLNKDLEVDGHFVTMQIWDTAGQERFRSLRTPFYRGSDCCLLTFSVDDSQSFQNLSNWKKEFIYYADVKEPESFPFVILGNKIDISERQVSTEEAQAWCRDNGDYPYFETSAKDATNVAAAFEEAVRRVLATEDRSDHLIQTDTVNLHRKPKPSSSCC
Gene ID
Swiss Prot
Synonyms	RAB9; RAB9A
Calculated MW	23kDa
Observed MW	23kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	SH-SY5Y, HeLa, HepG2, 293T, Mouse brain
Cellular location	Cell membrane, Cytoplasmic side, Cytoplasmic vesicle, Endoplasmic reticulum membrane, Golgi apparatus membrane, Late endosome, Lipid-anchor, phagosome, phagosome membrane
Customer validation	.(Mus musculus)