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RCCD1 Rabbit pAb (A17804)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Rat

ABclonal:Western blot - RCCD1 Rabbit pAb (A17804)

Western blot analysis of lysates from THP-1 cells, using RCCD1 Rabbit pAb (A17804) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product nameRCCD1 Rabbit pAb
Catalog No.A17804
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to be involved in chromatin organization. Located in cytosol and plasma membrane.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 230-360 of human RCCD1 (NP_001017919.1).
SequenceDIYIWGWNESGQLALPTRNLAEDGETVAREATELNEDGSQVKRTGGAEDGAPAPFIAVQPFPALLDLPMGSDAVKASCGSRHTAVVTRTGELYTWGWGKYGQLGHEDTTSLDRPRRVEYFVDKQLQVKAVT
Gene ID
Swiss Prot
SynonymsRCCD1
Calculated MW40kDa
Observed MW45kDa
ReactivityHuman, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesTHP-1
Cellular locationcytosol, plasma membrane
    ABclonal:Western blot - RCCD1 Rabbit pAb (A17804)}

    Western blot - RCCD1 Rabbit pAb (A17804)

    Western blot analysis of lysates from THP-1 cells, using RCCD1 Rabbit pAb (A17804) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)