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Rhodopsin Rabbit pAb (A15093)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Western blot - Rhodopsin Rabbit pAb (A15093)

Western blot analysis of various lysates using Rhodopsin Rabbit pAb (A15093) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - Rhodopsin Rabbit pAb (A15093)

Immunohistochemistry analysis of paraffin-embedded mouse retina using Rhodopsin Rabbit pAb (A15093) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameRhodopsin Rabbit pAb
Catalog No.A15093
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is found in rod cells in the back of the eye and is essential for vision in low-light conditions. The encoded protein binds to 11-cis retinal and is activated when light hits the retinal molecule. Defects in this gene are a cause of congenital stationary night blindness.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 200 to the C-terminus of human Rhodopsindopsin (NP_000530.1).
SequenceNESFVIYMFVVHFTIPMIIIFFCYGQLVFTVKEAAAQQQESATTQKAEKEVTRMVIIMVIAFLICWVPYASVAFYIFTHQGSNFGPIFMTIPAFFAKSAAIYNPVIYIMMNKQFRNCMLTTICCGKNPLGDDEASATVSKTETSQVAPA
Gene ID
Swiss Prot
SynonymsRP4; OPN2; CSNBAD1; Rhodopsin
Calculated MW39kDa
Observed MW39kDa
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesmouse eye, rat eye
Cellular locationMembrane, Multi-pass membrane protein
Customer validation

IHC(Mus musculus)

Documents

Certificate of Compliance

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ABclonal:Western blot - Rhodopsin Rabbit pAb (A15093)}

Western blot - Rhodopsin Rabbit pAb (A15093)

Western blot analysis of various lysates using Rhodopsin Rabbit pAb (A15093) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - Rhodopsin Rabbit pAb (A15093)}

Immunohistochemistry - Rhodopsin Rabbit pAb (A15093)

Immunohistochemistry analysis of paraffin-embedded mouse retina using Rhodopsin Rabbit pAb (A15093) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A15093? Please let us know so that we can cite the reference in this datasheet.

항체 (1)

Secondary Antibodies (26)