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SATB1 Rabbit mAb (A23147)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - SATB1 Rabbit mAb (A23147)

Western blot analysis of various lysates, using SATB1 Rabbit mAb (A23147) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - SATB1 Rabbit mAb (A23147)

Immunohistochemistry analysis of SATB1 in paraffin-embedded rat colon tissue using SATB1 Rabbit mAb (A23147) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - SATB1 Rabbit mAb (A23147)

Immunohistochemistry analysis of SATB1 in paraffin-embedded rat spleen tissue using SATB1 Rabbit mAb (A23147) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - SATB1 Rabbit mAb (A23147)

Immunohistochemistry analysis of SATB1 in paraffin-embedded mouse spleen tissue using SATB1 Rabbit mAb (A23147) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - SATB1 Rabbit mAb (A23147)

Immunohistochemistry analysis of SATB1 in paraffin-embedded mouse colon tissue using SATB1 Rabbit mAb (A23147) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - SATB1 Rabbit mAb (A23147)

Immunofluorescence analysis of paraffin-embedded mouse thymus using SATB1 Rabbit mAb (A23147) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

ABclonal:Immunofluorescence - SATB1 Rabbit mAb (A23147)

Immunofluorescence analysis of paraffin-embedded rat thymus using SATB1 Rabbit mAb (A23147) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Overview

Product nameSATB1 Rabbit mAb
Catalog No.A23147
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC56265
This gene encodes a matrix protein which binds nuclear matrix and scaffold-associating DNAs through a unique nuclear architecture. The protein recruits chromatin-remodeling factors in order to regulate chromatin structure and gene expression.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 572-763 of human SATB1 (NP_002962.1) .
SequenceNAVHHHGDRPPHIIHVPAEQIQQQQQQQQQQQQQQQAPPPPQPQQQPQTGPRLPPRQPTVASPAESDEENRQKTRPRTKISVEALGILQSFIQDVGLYPDEEAIQTLSAQLDLPKYTIIKFFQNQRYYLKHHGKLKDNSGLEVDVAEYKEEELLKDLEESVQDKNTNTLFSVKLEEELSVEGNTDINTDLKD
Gene ID
Swiss Prot
SynonymsDEFDA; KTZSL; SATB1
Calculated MW86kDa
Observed MW100kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesU-937
Cellular locationNucleus, Nucleus matrix, PML body

Documents

Certificate of Compliance

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    ABclonal:Western blot - SATB1 Rabbit mAb (A23147)}

    Western blot - SATB1 Rabbit mAb (A23147)

    Western blot analysis of various lysates, using SATB1 Rabbit mAb (A23147) at 1:2000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 60s.
    ABclonal:Immunohistochemistry - SATB1 Rabbit mAb (A23147)}

    Immunohistochemistry - SATB1 Rabbit mAb (A23147)

    Immunohistochemistry analysis of SATB1 in paraffin-embedded rat colon tissue using SATB1 Rabbit mAb (A23147) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - SATB1 Rabbit mAb (A23147)}

    Immunohistochemistry - SATB1 Rabbit mAb (A23147)

    Immunohistochemistry analysis of SATB1 in paraffin-embedded rat spleen tissue using SATB1 Rabbit mAb (A23147) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - SATB1 Rabbit mAb (A23147)}

    Immunohistochemistry - SATB1 Rabbit mAb (A23147)

    Immunohistochemistry analysis of SATB1 in paraffin-embedded mouse spleen tissue using SATB1 Rabbit mAb (A23147) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - SATB1 Rabbit mAb (A23147)}

    Immunohistochemistry - SATB1 Rabbit mAb (A23147)

    Immunohistochemistry analysis of SATB1 in paraffin-embedded mouse colon tissue using SATB1 Rabbit mAb (A23147) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunofluorescence - SATB1 Rabbit mAb (A23147)}

    Immunofluorescence - SATB1 Rabbit mAb (A23147)

    Immunofluorescence analysis of paraffin-embedded mouse thymus using SATB1 Rabbit mAb (A23147) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
    ABclonal:Immunofluorescence - SATB1 Rabbit mAb (A23147)}

    Immunofluorescence - SATB1 Rabbit mAb (A23147)

    Immunofluorescence analysis of paraffin-embedded rat thymus using SATB1 Rabbit mAb (A23147) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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