제품 > 항체 > 다중클론항체(pAb)

SATB1 Rabbit pAb (A5800)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from Jurkat cells, using SATB1 Rabbit pAb (A5800) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - SATB1 Rabbit pAb (A5800)

Immunohistochemistry analysis of paraffin-embedded Rat brain using SATB1 Rabbit pAb (A5800) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat spleen using SATB1 Rabbit pAb (A5800) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - SATB1 Rabbit pAb (A5800)

Immunofluorescence analysis of paraffin-embedded rat thymus using SATB1 Rabbit pAb (A5800) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - SATB1 Rabbit pAb (A5800)

Immunoprecipitation analysis of 300 μg extracts of Jurkat cells using 3 μg SATB1 antibody (A5800). Western blot was performed from the immunoprecipitate using SATB1 antibody (A5800) at a dilution of 1:500.

Overview

Product name	SATB1 Rabbit pAb
Catalog No.	A5800
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a matrix protein which binds nuclear matrix and scaffold-associating DNAs through a unique nuclear architecture. The protein recruits chromatin-remodeling factors in order to regulate chromatin structure and gene expression.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 572-763 of human SATB1 (NP_002962.1).
Sequence	NAVHHHGDRPPHIIHVPAEQIQQQQQQQQQQQQQQQAPPPPQPQQQPQTGPRLPPRQPTVASPAESDEENRQKTRPRTKISVEALGILQSFIQDVGLYPDEEAIQTLSAQLDLPKYTIIKFFQNQRYYLKHHGKLKDNSGLEVDVAEYKEEELLKDLEESVQDKNTNTLFSVKLEEELSVEGNTDINTDLKD
Gene ID
Swiss Prot
Synonyms	DEFDA; KTZSL; SATB1
Calculated MW	86kDa
Observed MW	100kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation
Positive samples	Jurkat
Cellular location	Nucleus, Nucleus matrix, PML body.
Customer validation	WB(Homo sapiens) IHC(Homo sapiens) IHC(Rattus norvegicus) IF(Homo sapiens)