제품 > 항체 > 다중클론항체(pAb)

SDG724 Rabbit pAb (A20704)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Oryza sativa

ABclonal:Western blot - SDG724 Rabbit pAb (A20704)

Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using SDG724 Rabbit pAb (A20704) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Overview

Product nameSDG724 Rabbit pAb
Catalog No.A20704
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The Histone Methyltransferase SDG724 Mediates H3K36me2/3 Deposition at MADS50 and RFT1 and Promotes Flowering in Rice.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-163 of Oryza sativa SDG724. (XP_015651319.1).
SequenceMIGRYGHPPNPLLLLRPHLASFLRRLPPLRHCSLAYGLELVADPRHRRDLCLPPMPRPAKIRKKHENVFDQLIKAIKAPVDFDLPPVLKEWKSNYYVPIKRNAYITRKRVEDDGIFCSCTPSGSSATCDKDCQCGMLFSCCSSTCKCENKCANKPFQHRTLRK
Gene ID
Swiss Prot
SynonymsSDG724; lvp1; OsSET34
Calculated MW45kDa
Observed MW70kDa
ReactivityOryza sativa
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesstems, spikes
Cellular location
    ABclonal:Western blot - SDG724 Rabbit pAb (A20704)}

    Western blot - SDG724 Rabbit pAb (A20704)

    Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using SDG724 Rabbit pAb (A20704) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)