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SEC61B Rabbit pAb (A21807)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - SEC61B Rabbit pAb (A21807)

Western blot analysis of various lysates using SEC61B Rabbit pAb (A21807) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Immunofluorescence - SEC61B Rabbit pAb (A21807)

Immunofluorescence analysis of NIH/3T3 cells using SEC61B Rabbit pAb (A21807) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - SEC61B Rabbit pAb (A21807)

Immunofluorescence analysis of PC-12 cells using SEC61B Rabbit pAb (A21807) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - SEC61B Rabbit pAb (A21807)

Immunofluorescence analysis of U2OS cells using SEC61B Rabbit pAb (A21807) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameSEC61B Rabbit pAb
Catalog No.A21807
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The Sec61 complex is the central component of the protein translocation apparatus of the endoplasmic reticulum (ER) membrane. Oligomers of the Sec61 complex form a transmembrane channel where proteins are translocated across and integrated into the ER membrane. This complex consists of three membrane proteins- alpha, beta, and gamma. This gene encodes the beta-subunit protein. The Sec61 subunits are also observed in the post-ER compartment, suggesting that these proteins can escape the ER and recycle back. There is evidence for multiple polyadenylated sites for this transcript.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-96 of human SEC61B (NP_006799.1).
SequenceMPGPTPSGTNVGSSGRSPSKAVAARAAGSTVRQRKNASCGTRSAGRTTSAGTGGMWRFYTEDSPGLKVGPVPVLVMSLLFIASVFMLHIWGKYTRS
Gene ID
Swiss Prot
SynonymsSEC61B; Sec61 translocon beta subunit
Calculated MW10kDa
Observed MWRefer to figures
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHeLa, Hep G2, SH-SY5Y, Mouse stomach, Rat liver, Rat stomach;
Cellular locationEndoplasmic reticulum membrane, Single-pass membrane protein

Documents

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ABclonal:Western blot - SEC61B Rabbit pAb (A21807)}

Western blot - SEC61B Rabbit pAb (A21807)

Western blot analysis of various lysates using SEC61B Rabbit pAb (A21807) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Immunofluorescence - SEC61B Rabbit pAb (A21807)}

Immunofluorescence - SEC61B Rabbit pAb (A21807)

Immunofluorescence analysis of NIH/3T3 cells using SEC61B Rabbit pAb (A21807) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - SEC61B Rabbit pAb (A21807)}

Immunofluorescence - SEC61B Rabbit pAb (A21807)

Immunofluorescence analysis of PC-12 cells using SEC61B Rabbit pAb (A21807) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - SEC61B Rabbit pAb (A21807)}

Immunofluorescence - SEC61B Rabbit pAb (A21807)

Immunofluorescence analysis of U2OS cells using SEC61B Rabbit pAb (A21807) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (1)

Secondary Antibodies (26)