제품 > 항체 > 다중클론항체(pAb)

SEMA4C Rabbit pAb (A8225)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - SEMA4C Rabbit pAb (A8225)

Western blot analysis of various lysates using SEMA4C Rabbit pAb (A8225) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product nameSEMA4C Rabbit pAb
Catalog No.A8225
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable chemorepellent activity and semaphorin receptor binding activity. Involved in muscle cell differentiation and positive regulation of stress-activated MAPK cascade. Located in extracellular space.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 749-833 of human SEMA4C (NP_060259.4).
SequenceHARCQPGGGPPSPPPGIPGQPLPSPTRLHLGGGRNSNANGYVRLQLGGEDRGGLGHPLPELADELRRKLQQRQPLPDSNPEESSV
Gene ID
Swiss Prot
SynonymsSEMAF; SEMAI; SEMACL1; M-SEMA-F; SEMA4C
Calculated MW93kDa
Observed MW110kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMCF7, BT-474, Mouse heart
Cellular locationCell junction, Cytoplasmic vesicle, Single-pass type I membrane protein, postsynaptic cell membrane, postsynaptic density, secretory vesicle, synapse, synaptic vesicle membrane

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ABclonal:Western blot - SEMA4C Rabbit pAb (A8225)}

Western blot - SEMA4C Rabbit pAb (A8225)

Western blot analysis of various lysates using SEMA4C Rabbit pAb (A8225) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)