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SET/TAF1 Rabbit mAb (A9173)

Publication (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - SET/TAF1 Rabbit mAb (A9173)

Western blot analysis of various lysates using SET/TAF1 Rabbit mAb (A9173) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - SET/TAF1 Rabbit mAb (A9173)

Immunohistochemistry analysis of paraffin-embedded Rat brain using SET/TAF1 Rabbit mAb (A9173) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - SET/TAF1 Rabbit mAb (A9173)

Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using SET/TAF1 Rabbit mAb (A9173) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - SET/TAF1 Rabbit mAb (A9173)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen using SET/TAF1 Rabbit mAb (A9173) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - SET/TAF1 Rabbit mAb (A9173)

Immunofluorescence analysis of U-2 OS cells using SET/TAF1 Rabbit mAb (A9173) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - SET/TAF1 Rabbit mAb (A9173)

Immunoprecipitation analysis of 300 μg extracts of 293T cells using 3 μg SET/TAF1 antibody (A9173). Western blot was performed from the immunoprecipitate using SET/TAF1 antibody (A9173) at a dilution of 1:1000.

Overview

Product nameSET/TAF1 Rabbit mAb
Catalog No.A9173
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1462
The protein encoded by this gene inhibits acetylation of nucleosomes, especially histone H4, by histone acetylases (HAT). This inhibition is most likely accomplished by masking histone lysines from being acetylated, and the consequence is to silence HAT-dependent transcription. The encoded protein is part of a complex localized to the endoplasmic reticulum but is found in the nucleus and inhibits apoptosis following attack by cytotoxic T lymphocytes. This protein can also enhance DNA replication of the adenovirus genome. Several transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 200-290 of human SET/TAF1 (Q01105).
SequenceESFFTWFTDHSDAGADELGEVIKDDIWPNPLQYYLVPDMDDEEGEGEEDDDDDEEEEGLEDIDEEGDEDEGEEDEDDDEGEEGEEDEGEDD
Gene ID
Swiss Prot
Synonyms2PP2A; IGAAD; MRD58; TAF-I; I2PP2A; IPP2A2; PHAPII; TAF-IBETA; SET/TAF1
Calculated MW33kDa
Observed MW37kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samples293T, Mouse testis
Cellular locationCytoplasm, Endoplasmic reticulum, Nucleus, cytosol, nucleoplasm
Customer validation

WB(Mus musculus, Other)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - SET/TAF1 Rabbit mAb (A9173)}

Western blot - SET/TAF1 Rabbit mAb (A9173)

Western blot analysis of various lysates using SET/TAF1 Rabbit mAb (A9173) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - SET/TAF1 Rabbit mAb (A9173)}

Immunohistochemistry - SET/TAF1 Rabbit mAb (A9173)

Immunohistochemistry analysis of paraffin-embedded Rat brain using SET/TAF1 Rabbit mAb (A9173) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - SET/TAF1 Rabbit mAb (A9173)}

Immunohistochemistry - SET/TAF1 Rabbit mAb (A9173)

Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using SET/TAF1 Rabbit mAb (A9173) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - SET/TAF1 Rabbit mAb (A9173)}

Immunohistochemistry - SET/TAF1 Rabbit mAb (A9173)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen using SET/TAF1 Rabbit mAb (A9173) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - SET/TAF1 Rabbit mAb (A9173)}

Immunofluorescence - SET/TAF1 Rabbit mAb (A9173)

Immunofluorescence analysis of U-2 OS cells using SET/TAF1 Rabbit mAb (A9173) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - SET/TAF1 Rabbit mAb (A9173)}

Immunoprecipitation - SET/TAF1 Rabbit mAb (A9173)

Immunoprecipitation analysis of 300 μg extracts of 293T cells using 3 μg SET/TAF1 antibody (A9173). Western blot was performed from the immunoprecipitate using SET/TAF1 antibody (A9173) at a dilution of 1:1000.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A9173? Please let us know so that we can cite the reference in this datasheet.

항체 (3)

Secondary Antibodies (26)