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SET/TAF1 Rabbit pAb (A12502)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - SET/TAF1 Rabbit pAb (A12502)

Western blot analysis of various lysates using SET/TAF1 Rabbit pAb (A12502) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - SET/TAF1 Rabbit pAb (A12502)

Immunohistochemistry analysis of paraffin-embedded rat kidney using SET/TAF1 Rabbit pAb (A12502) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - SET/TAF1 Rabbit pAb (A12502)

Immunohistochemistry analysis of paraffin-embedded human esophagus using SET/TAF1 Rabbit pAb (A12502) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - SET/TAF1 Rabbit pAb (A12502)

Immunohistochemistry analysis of paraffin-embedded mouse brain using SET/TAF1 Rabbit pAb (A12502) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameSET/TAF1 Rabbit pAb
Catalog No.A12502
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene inhibits acetylation of nucleosomes, especially histone H4, by histone acetylases (HAT). This inhibition is most likely accomplished by masking histone lysines from being acetylated, and the consequence is to silence HAT-dependent transcription. The encoded protein is part of a complex localized to the endoplasmic reticulum but is found in the nucleus and inhibits apoptosis following attack by cytotoxic T lymphocytes. This protein can also enhance DNA replication of the adenovirus genome. Several transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 146-277 of human SET/TAF1 (NP_003002.2).
SequenceNESGDPSSKSTEIKWKSGKDLTKRSSQTQNKASRKRQHEEPESFFTWFTDHSDAGADELGEVIKDDIWPNPLQYYLVPDMDDEEGEGEEDDDDDEEEEGLEDIDEEGDEDEGEEDEDDDEGEEGEEDEGEDD
Gene ID
Swiss Prot
Synonyms2PP2A; IGAAD; MRD58; TAF-I; I2PP2A; IPP2A2; PHAPII; TAF-IBETA; SET/TAF1
Calculated MW33kDa
Observed MW34kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHeLa, 293T, BT-474, HL-60, NIH/3T3, Mouse spleen
Cellular locationCytoplasm, Endoplasmic reticulum, Nucleus, cytosol, nucleoplasm

Documents

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ABclonal:Western blot - SET/TAF1 Rabbit pAb (A12502)}

Western blot - SET/TAF1 Rabbit pAb (A12502)

Western blot analysis of various lysates using SET/TAF1 Rabbit pAb (A12502) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - SET/TAF1 Rabbit pAb (A12502)}

Immunohistochemistry - SET/TAF1 Rabbit pAb (A12502)

Immunohistochemistry analysis of paraffin-embedded rat kidney using SET/TAF1 Rabbit pAb (A12502) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - SET/TAF1 Rabbit pAb (A12502)}

Immunohistochemistry - SET/TAF1 Rabbit pAb (A12502)

Immunohistochemistry analysis of paraffin-embedded human esophagus using SET/TAF1 Rabbit pAb (A12502) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - SET/TAF1 Rabbit pAb (A12502)}

Immunohistochemistry - SET/TAF1 Rabbit pAb (A12502)

Immunohistochemistry analysis of paraffin-embedded mouse brain using SET/TAF1 Rabbit pAb (A12502) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (3)

Secondary Antibodies (26)