제품 > 항체 > 다중클론항체(pAb)

SH2D2A Rabbit pAb (A18401)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - SH2D2A Rabbit pAb (A18401)

Western blot analysis of various lysates using SH2D2A Rabbit pAb (A18401) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Western blot - SH2D2A Rabbit pAb (A18401)

Western blot analysis of various lysates using SH2D2A Rabbit pAb (A18401) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Overview

Product nameSH2D2A Rabbit pAb
Catalog No.A18401
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes an adaptor protein thought to function in T-cell signal transduction. A related protein in mouse is responsible for the activation of lymphocyte-specific protein-tyrosine kinase and functions in downstream signaling. Alternative splicing results in multiple transcript variants.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 250-350 of human SH2D2A (NP_003966.2).
SequencePAKPQLPPEVYTIPVPRHRPAPRPKPSNPIYNEPDEPIAFYAMGRGSPGEAPSNIYVEVEDEGLPATLGHPVLRKSWSRPVPGGQNTGGSQLHSENSVIGQ
Gene ID
Swiss Prot
SynonymsSCAP; TSAD; VRAP; F2771; SH2D2A
Calculated MW43kDa
Observed MW41kDa/43kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesU-87MG, K-562, Mouse spleen, Rat thymus
Cellular locationcytoplasm, cytosol
Customer validation

RT-PCR(Mus musculus)

WB(Mus musculus)

ABclonal:Western blot - SH2D2A Rabbit pAb (A18401)}

Western blot - SH2D2A Rabbit pAb (A18401)

Western blot analysis of various lysates using SH2D2A Rabbit pAb (A18401) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - SH2D2A Rabbit pAb (A18401)}

Western blot - SH2D2A Rabbit pAb (A18401)

Western blot analysis of various lysates using SH2D2A Rabbit pAb (A18401) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

* For research use only. Not for therapeutic or diagnostic purposes.

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