제품 > 항체 > 다중클론항체(pAb)

SH3BGRL Rabbit pAb (A17530)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using SH3BGRL Rabbit pAb (A17530) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - SH3BGRL Rabbit pAb (A17530)

Immunofluorescence analysis of U-2 OS cells using SH3BGRL Rabbit pAb (A17530) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	SH3BGRL Rabbit pAb
Catalog No.	A17530
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Predicted to enable SH3 domain binding activity. Located in extracellular exosome.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-114 of human SH3BGRL (NP_003013.1).
Sequence	MVIRVYIASSSGSTAIKKKQQDVLGFLEANKIGFEEKDIAANEENRKWMRENVPENSRPATGYPLPPQIFNESQYRGDYDAFFEARENNAVYAFLGLTAPPGSKEAEVQAKQQA
Gene ID
Swiss Prot
Synonyms	SH3BGR; HEL-S-115; SH3BGRL
Calculated MW	13kDa
Observed MW	16kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	Mouse lung, Mouse spleen, Mouse liver, Rat lung, Rat liver
Cellular location	cytoplasm, cytosol, extracellular exosome, extracellular space, nucleus, plasma membrane