Review (1)Publications (11) Datasheet
Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using SIRT3 Rabbit pAb (A7307) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse kidney using SIRT3 Rabbit pAb (A7307) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat kidney using SIRT3 Rabbit pAb (A7307) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of NIH/3T3 cells using SIRT3 Rabbit pAb (A7307) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
| Product name | SIRT3 Rabbit pAb |
|---|---|
| Catalog No. | A7307 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 388-399 of human SIRT3 (NP_036371.1). |
|---|---|
| Sequence | QRETGKLDGPDK |
| Gene ID | |
| Swiss Prot | |
| Synonyms | SIR2L3; SIRT3 |
| Calculated MW | 44kDa |
| Observed MW | 28kDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3. |
| Key application | Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation |
| Positive samples | |
| Cellular location | Mitochondrion matrix. |
| Customer validation | (769-P PLVX;769-P SIRT3 OE) WB(Rattus norvegicus, Mus musculus, Homo sapiens) IF(Mus musculus) IHC(Homo sapiens) IF(Homo sapiens) IHC(Homo sapiens,Mus musculus) WB(Homo sapiens,Mus musculus) |
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