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SLC25A23 Rabbit pAb (A14352)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - SLC25A23 Rabbit pAb (A14352)

Western blot analysis of various lysates using SLC25A23 Rabbit pAb (A14352) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Immunofluorescence - SLC25A23 Rabbit pAb (A14352)

Immunofluorescence analysis of NIH/3T3 cells using SLC25A23 Rabbit pAb (A14352) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameSLC25A23 Rabbit pAb
Catalog No.A14352
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable ATP transmembrane transporter activity. Involved in calcium import into the mitochondrion; positive regulation of mitochondrial calcium ion concentration; and regulation of cellular hyperosmotic salinity response. Located in mitochondrion.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human SLC25A23 (NP_077008.2).
SequenceMRGSPGDAERRQRWGRLFEELDSNKDGRVDVHELRQGLARLGGGNPDPGAQQGISSEGDADPDGGLDLEEFSRYLQEREQRLLLMFHSLDRNQDGHIDVSEIQQSFRALGISISLEQAEKILHSMDRDGTMTIDWQEWRDHFLLHSLENVEDVLYFWKHS
Gene ID
Swiss Prot
SynonymsAPC2; MCSC2; SCAMC3; SCaMC-3; SLC25A23
Calculated MW52kDa
Observed MW52kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHeLa, 293T, Mouse pancreas, Mouse testis, Mouse brain, Mouse kidney, Rat testis, Rat brain
Cellular locationMitochondrion inner membrane, Multi-pass membrane protein

Documents

Certificate of Compliance

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    ABclonal:Western blot - SLC25A23 Rabbit pAb (A14352)}

    Western blot - SLC25A23 Rabbit pAb (A14352)

    Western blot analysis of various lysates using SLC25A23 Rabbit pAb (A14352) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 3s.
    ABclonal:Immunofluorescence - SLC25A23 Rabbit pAb (A14352)}

    Immunofluorescence - SLC25A23 Rabbit pAb (A14352)

    Immunofluorescence analysis of NIH/3T3 cells using SLC25A23 Rabbit pAb (A14352) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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    Secondary Antibodies (26)