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SMARCA2 / BRM Rabbit mAb (A23291)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of various lysates, using SMARCA2 / BRM Rabbit mAb (A23291) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - SMARCA2 / BRM Rabbit mAb (A23291)

Immunohistochemistry analysis of paraffin-embedded human cervix cancer using SMARCA2 / BRM Rabbit mAb (A23291) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - SMARCA2 / BRM Rabbit mAb (A23291)

Immunofluorescence analysis of HeLa cells using SMARCA2 / BRM Rabbit mAb (A23291) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - SMARCA2 / BRM Rabbit mAb (A23291)

Immunoprecipitation analysis of 300 μg extracts of Hela cells using 3 μg SMARCA2 / BRM Rabbit mAb (A23291). Western blot was performed from the immunoprecipitate using SMARCA2 / BRM Rabbit mAb (A23291) at a dilition of 1:5000.

Overview

Product name	SMARCA2 / BRM Rabbit mAb
Catalog No.	A23291
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC59944

Background

The protein encoded by this gene is a member of the SWI/SNF family of proteins and is highly similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene, which contains a trinucleotide repeat (CAG) length polymorphism.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 201-300 of human SMARCA2 (NP_003061.3).
Sequence	TLQLAVQGKRTLPGLQQQQQQQQQQQQQQQQQQQQQQQPQQQPPQPQTQQQQQPALVNYNRPSGPGPELSGPSTPQKLPVPAPGGRPSPAPPAAAQPPAA
Gene ID
Swiss Prot
Synonyms	BIS; BRM; SNF2; SWI2; hBRM; NCBRS; Sth1p; BAF190; SNF2L2; SNF2LA; hSNF2a; SMARCA2 / BRM
Calculated MW	181kDa
Observed MW	220kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation
Positive samples	HeLa
Cellular location	nucleus
Customer validation	IHC(Homo sapiens)