제품 > 항체 > 다중클론항체(pAb)

SMU1 Rabbit pAb (A9110)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - SMU1 Rabbit pAb (A9110)

Western blot analysis of various lysates using SMU1 Rabbit pAb (A9110) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Overview

Product nameSMU1 Rabbit pAb
Catalog No.A9110
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Involved in mRNA splicing, via spliceosome. Located in nucleus. Part of U2-type precatalytic spliceosome.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human SMU1 (NP_060695.2).
SequenceMSIEIESSDVIRLIMQYLKENSLHRALATLQEETTVSLNTVDSIESFVADINSGHWDTVLQAIQSLKLPDKTLIDLYEQVVLELIELRELGAARSLLRQTDPMIMLKQTQPERYIHLENLLARSYFDPREAYPDGSSKEKRRAAIAQALAGEVSVVPPSRLMALLGQALKWQQHQGLLPPGMTIDLFRGKAAVKDVEEEKFPTQLSRHIKFGQKSHVECA
Gene ID
Swiss Prot
SynonymsBWD; SMU-1; fSAP57; SMU1
Calculated MW58kDa
Observed MW50kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:200 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa, A-431, HT-29, Mouse liver, Mouse intestine
Cellular locationCytoplasm, Nucleus

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    ABclonal:Western blot - SMU1 Rabbit pAb (A9110)}

    Western blot - SMU1 Rabbit pAb (A9110)

    Western blot analysis of various lysates using SMU1 Rabbit pAb (A9110) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)