제품 > 항체 > 다중클론항체(pAb)

SNRNP35 Rabbit pAb (A13211)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - SNRNP35 Rabbit pAb (A13211)

Western blot analysis of various lysates using SNRNP35 Rabbit pAb (A13211) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Overview

Product nameSNRNP35 Rabbit pAb
Catalog No.A13211
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a homolog of the U1-snRNP binding protein. The N-terminal half contains a RNA recognition motif and the C-terminal half is rich in Arg/Asp and Arg/Glu dipeptides, which is a characteristic of a variety of splicing factors. This protein is a component of the U11/U12 small nuclear ribonucleoproteins (snRNP) that form part of the U12-type spliceosome. Alternative splicing results in multiple transcript variants encoding two distinct isoforms and representing a non-protein coding variant.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human SNRNP35 (NP_073208.1).
SequenceMNDWMPIAKEYDPLKAGSIDGTDEDPHDRAVWRAMLARYVPNKGVIGDPLLTLFVARLNLQTKEDKLKEVFSRYGDIRRLRLVRDLVTGFSKGYAFIEYKEERAVIKAYRDADGLVIDQHEIFVDYELERTLKGWIPRRLGGGLGGKKES
Gene ID
Swiss Prot
SynonymsHM1; HM-1; U1SNRNPBP; SNRNP35
Calculated MW29kDa
Observed MW35kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesU-87MG, Mouse brain
Cellular locationNucleus
    ABclonal:Western blot - SNRNP35 Rabbit pAb (A13211)}

    Western blot - SNRNP35 Rabbit pAb (A13211)

    Western blot analysis of various lysates using SNRNP35 Rabbit pAb (A13211) at 1:3000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.

    * For research use only. Not for therapeutic or diagnostic purposes.