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SNRPA1 Rabbit pAb (A12161)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - SNRPA1 Rabbit pAb (A12161)

Western blot analysis of various lysates using SNRPA1 Rabbit pAb (A12161) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - SNRPA1 Rabbit pAb (A12161)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using SNRPA1 Rabbit pAb (A12161) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - SNRPA1 Rabbit pAb (A12161)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using SNRPA1 Rabbit pAb (A12161) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - SNRPA1 Rabbit pAb (A12161)

Immunohistochemistry analysis of paraffin-embedded mouse liver using SNRPA1 Rabbit pAb (A12161) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - SNRPA1 Rabbit pAb (A12161)

Immunohistochemistry analysis of paraffin-embedded rat liver using SNRPA1 Rabbit pAb (A12161) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - SNRPA1 Rabbit pAb (A12161)

Immunohistochemistry analysis of paraffin-embedded rat lung using SNRPA1 Rabbit pAb (A12161) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Overview

Product nameSNRPA1 Rabbit pAb
Catalog No.A12161
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables RNA binding activity. Involved in mRNA splicing, via spliceosome and spermatogenesis. Located in nuclear speck. Part of U2-type catalytic step 2 spliceosome and U2-type precatalytic spliceosome. Implicated in connective tissue disease.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human SNRPA1 (NP_003081.2).
SequenceMVKLTAELIEQAAQYTNAVRDRELDLRGYKIPVIENLGATLDQFDAIDFSDNEIRKLDGFPLLRRLKTLLVNNNRICRIGEGLDQALPCLTELILTNNSLVELGDLDPLASLKSLTYLSILRNPVTNKKHYRLYVIYKVPQVRVLDFQKVKLKERQEAEKMFKGKRGAQLAKDIARRSKTFNPGAGLPTDKKKGGPSPGDVEAIKNAIANASTLAEVERL
Gene ID
Swiss Prot
SynonymsLea1; U2A'; SNRPA1
Calculated MW28kDa
Observed MW28kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHT-29, Jurkat, Raji, HeLa, NIH/3T3, Mouse kidney, Mouse liver, Mouse lung
Cellular locationNucleus

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - SNRPA1 Rabbit pAb (A12161)}

    Western blot - SNRPA1 Rabbit pAb (A12161)

    Western blot analysis of various lysates using SNRPA1 Rabbit pAb (A12161) at 1:3000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Immunohistochemistry - SNRPA1 Rabbit pAb (A12161)}

    Immunohistochemistry - SNRPA1 Rabbit pAb (A12161)

    Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using SNRPA1 Rabbit pAb (A12161) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - SNRPA1 Rabbit pAb (A12161)}

    Immunohistochemistry - SNRPA1 Rabbit pAb (A12161)

    Immunohistochemistry analysis of paraffin-embedded human lung cancer using SNRPA1 Rabbit pAb (A12161) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - SNRPA1 Rabbit pAb (A12161)}

    Immunohistochemistry - SNRPA1 Rabbit pAb (A12161)

    Immunohistochemistry analysis of paraffin-embedded mouse liver using SNRPA1 Rabbit pAb (A12161) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - SNRPA1 Rabbit pAb (A12161)}

    Immunohistochemistry - SNRPA1 Rabbit pAb (A12161)

    Immunohistochemistry analysis of paraffin-embedded rat liver using SNRPA1 Rabbit pAb (A12161) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - SNRPA1 Rabbit pAb (A12161)}

    Immunohistochemistry - SNRPA1 Rabbit pAb (A12161)

    Immunohistochemistry analysis of paraffin-embedded rat lung using SNRPA1 Rabbit pAb (A12161) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

    * For research use only. Not for therapeutic or diagnostic purposes.

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    Secondary Antibodies (26)