제품 > 항체 > 단일클론항체(mAb)

SOD1 Rabbit mAb (A12537)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using SOD1 Rabbit mAb (A12537) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product name	SOD1 Rabbit mAb
Catalog No.	A12537
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC51789

Background

The protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. In addition, this protein contains an antimicrobial peptide that displays antibacterial, antifungal, and anti-MRSA activity against E. coli, E. faecalis, S. aureus, S. aureus MRSA LPV+, S. agalactiae, and yeast C. krusei. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 2-154 of human SOD1 (NP_000445.1).
Sequence	ATKAVCVLKGDGPVQGIINFEQKESNGPVKVWGSIKGLTEGLHGFHVHEFGDNTAGCTSAGPHFNPLSRKHGGPKDEERHVGDLGNVTADKDGVADVSIEDSVISLSGDHCIIGRTLVVHEKADDLGKGGNEESTKTGNAGSRLACGVIGIAQ
Gene ID
Swiss Prot
Synonyms	ALS; SOD; ALS1; IPOA; STAHP; hSod1; HEL-S-44; homodimer; SOD1
Calculated MW	16kDa
Observed MW	16kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:6000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	HeLa, 293T, MCF7, Mouse liver, Mouse ovary, Mouse brain, Rat lung, Rat liver, Rat brain
Cellular location	Cytoplasm, Nucleus.
Customer validation	WB(Mus musculus, Rattus norvegicus, Homo sapiens) IF(Mus musculus) RT-qPCR(Homo sapiens) WB(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

SOD1 Rabbit mAb images

Western blot - SOD1 Rabbit mAb (A12537)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for SOD1 Rabbit mAb (A12537)

Product:

SOD1 Rabbit mAb

Journal:

Free Radic Biol Med

Application:

IF:

7.1

Species:

Mus musculus

PMID:

34097996

Title:

Induction of monoamine oxidase A-mediated oxidative stress and impairment of NRF2-antioxidant defence response by polyphenol-rich fraction of Bergenia ligulata sensitizes prostate cancer cells in vitro and in vivo

References for SOD1 Rabbit mAb (A12537)

Product:

SOD1 Rabbit mAb

Journal:

Eur J Pharmacol

Application:

IF:

4.2

Species:

Rattus norvegicus

PMID:

34280397

Title:

Thymosin beta 4 alleviates non-alcoholic fatty liver by inhibiting ferroptosis via up-regulation of GPX4 0

References for SOD1 Rabbit mAb (A12537)

Product:

SOD1 Rabbit mAb

Journal:

Human molecular genetics

Application:

IF:

3.1

Species:

Homo sapiens

PMID:

35567411

Title:

Leber's hereditary optic neuropathy (LHON)-associated ND6 14 484 T > C mutation caused pleiotropic effects on the complex I, RNA homeostasis, apoptosis and mitophagy

References for SOD1 Rabbit mAb (A12537)

Product:

SOD1 Rabbit mAb

Journal:

Hypertension research : official journal of the Japanese Society of Hypertension

Application:

IF:

4.3

Species:

Homo sapiens

PMID:

35585170

Title:

In utero hypoxia attenuated acetylcholine-mediated vasodilatation via CHRM3/p-NOS3 in fetal sheep MCA: role of ROS/ERK1/2

References for SOD1 Rabbit mAb (A12537)

Product:

SOD1 Rabbit mAb

Journal:

Nutrients

Application:

IF:

5.9

Species:

Mus musculus

PMID:

36839230

Title:

Anti-Obesity Effect of Auricularia delicate Involves Intestinal-Microbiota-Mediated Oxidative Stress Regulation in High-Fat-Diet-Fed Mice - PMC

References for SOD1 Rabbit mAb (A12537)

Product:

SOD1 Rabbit mAb

Journal:

International journal of biological macromolecules

Application:

IF:

7.7

Species:

Mus musculus

PMID:

37119895

Title:

Study of anti-fatigue activity of polysaccharide from fruiting bodies of Armillaria gallica

References for SOD1 Rabbit mAb (A12537)

Product:

SOD1 Rabbit mAb

Journal:

Nutrients

Application:

IF:

5.9

Species:

Rattus norvegicus

PMID:

37447162

Title:

Stingless Bee (Heterotrigona Itama) Honey and Its Phenolic-Rich Extract Ameliorate Oxidant-Antioxidant Balance via KEAP1-NRF2 Signalling Pathway - PMC

References for SOD1 Rabbit mAb (A12537)

Product:

SOD1 Rabbit mAb

Journal:

Journal of biomedical science

Application:

IF:

Species:

Homo sapiens

PMID:

37537557

Title:

Optimized allotopic expression of mitochondrial ND6 transgene restored complex I and apoptosis deficiencies caused by LHON-linked ND6 14484T>C mutation - PMC

References for SOD1 Rabbit mAb (A12537)

Product:

SOD1 Rabbit mAb

Journal:

Journal of agricultural and food chemistry

Application:

IF:

5.7

Species:

Homo sapiens

PMID:

37196215

Title:

Virtual Screening of Nrf2 Dietary-Derived Agonists and Safety by a New Deep-Learning Model and Verified In Vitro and In Vivo

References for SOD1 Rabbit mAb (A12537)

Product:

SOD1 Rabbit mAb

Journal:

Molecules (Basel, Switzerland)

Application:

IF:

4.6

Species:

Mus musculus

PMID:

38005205

Title:

Zaluzanin C Alleviates Inflammation and Lipid Accumulation in Kupffer Cells and Hepatocytes by Regulating Mitochondrial ROS - PMC

References for SOD1 Rabbit mAb (A12537)

Product:

SOD1 Rabbit mAb

Journal:

Application:

IF:

5.6

Species:

Rattus norvegicus

PMID:

38069169

Title:

Lactoferrin Alleviates Ethanol-Induced Injury via Promoting Nrf2 Nuclear Translocation in BRL-3A Rat Liver Cells - PMC

References for SOD1 Rabbit mAb (A12537)

Product:

SOD1 Rabbit mAb

Journal:

Toxicon : official journal of the International Society on Toxinology

Application:

IF:

2.6

Species:

Homo sapiens

PMID:

38030095

Title:

-eudesmol inhibits cell proliferation and induces ferroptosis via regulating MAPK signaling pathway in breast cancer

References for SOD1 Rabbit mAb (A12537)

Product:

SOD1 Rabbit mAb

Journal:

Molecular nutrition & food research

Application:

IF:

5.2

Species:

Mus musculus

PMID:

36738079

Title:

Oxidative Stress Mediated by N6Methyladenosine Methylation Contributes to HighFat Diet Induced Male Reproductive Dysfunction

References for SOD1 Rabbit mAb (A12537)

Product:

SOD1 Rabbit mAb

Journal:

BioMed research international

Application:

WB, IF

IF:

Species:

Mus musculus

PMID:

35686233

Title:

Metabolomics Analysis Reveals Alterations in Cochlear Metabolic Profiling in Mice with Noise-Induced Hearing Loss

References for SOD1 Rabbit mAb (A12537)

Product:

SOD1 Rabbit mAb

Journal:

International journal of biological macromolecules

Application:

IF:

7.7

Species:

Rattus norvegicus

PMID:

37553034

Title:

Fucoidan-functionalized gelatin methacryloyl microspheres ameliorate intervertebral disc degeneration by restoring redox and matrix homeostasis of nucleus

References for SOD1 Rabbit mAb (A12537)

Product:

SOD1 Rabbit mAb

Journal:

Scientific reports

Application:

IF:

3.8

Species:

Mus musculus

PMID:

38570541