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SOD1 Rabbit pAb (A0274)

Review (1)Publications (31) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - SOD1 Rabbit pAb (A0274)

Western blot analysis of various lysates using SOD1 Rabbit pAb (A0274) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - SOD1 Rabbit pAb (A0274)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using SOD1 Rabbit pAb (A0274) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - SOD1 Rabbit pAb (A0274)

Immunohistochemistry analysis of paraffin-embedded rat kidney using SOD1 Rabbit pAb (A0274) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - SOD1 Rabbit pAb (A0274)

Immunofluorescence analysis of NIH/3T3 cells using SOD1 Rabbit pAb (A0274) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - SOD1 Rabbit pAb (A0274)

Immunoprecipitation of SOD1 in 300 µg extracts from MCF7 cells using 3 µg SOD1 Rabbit pAb (A0274). Western blot analysis was performed using SOD1 Rabbit pAb (A0274) at 1:1000 dilution.

Review
ABclonal: review for SOD1 Rabbit pAb(A0274)

Overview

Product nameSOD1 Rabbit pAb
Catalog No.A0274
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. In addition, this protein contains an antimicrobial peptide that displays antibacterial, antifungal, and anti-MRSA activity against E. coli, E. faecalis, S. aureus, S. aureus MRSA LPV+, S. agalactiae, and yeast C. krusei. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 2-154 of human SOD1 (NP_000445.1).
SequenceATKAVCVLKGDGPVQGIINFEQKESNGPVKVWGSIKGLTEGLHGFHVHEFGDNTAGCTSAGPHFNPLSRKHGGPKDEERHVGDLGNVTADKDGVADVSIEDSVISLSGDHCIIGRTLVVHEKADDLGKGGNEESTKTGNAGSRLACGVIGIAQ
Gene ID
Swiss Prot
SynonymsALS; SOD; ALS1; IPOA; STAHP; hSod1; HEL-S-44; homodimer; SOD1
Calculated MW16kDa
Observed MW16-18kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesHeLa, 293T, MCF7, Mouse liver, Mouse brain, Rat lung, Rat liver, Rat brain
Cellular locationCytoplasm, Nucleus
Customer validation

(293T)

WB(Homo sapiens, Mus musculus, Bos taurus, Rattus norvegicus, Eriocheir sinensis H.Milne-Edwards, Other, Mus musculus)

IHC(Rattus norvegicus)

ELISA(Mus musculus)

IF(Ctenopharyngodon idellus)

Co-IP(Homo sapiens)

WB(Homo sapiens)

Co-IP(Mus musculus)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - SOD1 Rabbit pAb (A0274)}

    Western blot - SOD1 Rabbit pAb (A0274)

    Western blot analysis of various lysates using SOD1 Rabbit pAb (A0274) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Immunohistochemistry - SOD1 Rabbit pAb (A0274)}

    Immunohistochemistry - SOD1 Rabbit pAb (A0274)

    Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using SOD1 Rabbit pAb (A0274) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - SOD1 Rabbit pAb (A0274)}

    Immunohistochemistry - SOD1 Rabbit pAb (A0274)

    Immunohistochemistry analysis of paraffin-embedded rat kidney using SOD1 Rabbit pAb (A0274) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
    ABclonal:Immunofluorescence - SOD1 Rabbit pAb (A0274)}

    Immunofluorescence - SOD1 Rabbit pAb (A0274)

    Immunofluorescence analysis of NIH/3T3 cells using SOD1 Rabbit pAb (A0274) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunoprecipitation - SOD1 Rabbit pAb (A0274)}

    Immunoprecipitation - SOD1 Rabbit pAb (A0274)

    Immunoprecipitation of SOD1 in 300 µg extracts from MCF7 cells using 3 µg SOD1 Rabbit pAb (A0274). Western blot analysis was performed using SOD1 Rabbit pAb (A0274) at 1:1000 dilution.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Submit an review for A0274

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    Reviews List

    Publishing research using A0274? Please let us know so that we can cite the reference in this datasheet.

    항체 (2)

    SOD1 Rabbit mAb
    A12537

    WBELISA

    Reactivity:Human, Mouse, Rat

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    SOD1 Rabbit mAb
    A22594

    ELISAWBIHC-P

    Reactivity:Human

    SOD1 Rabbit mAb

    ELISA 키트 (1)

    Human Superoxide dismutase [Cu-Zn] (SOD1) ELISA Kit
    RK00263

    Human Superoxide dismutase [Cu-Zn] (SOD1) ELISA Kit

    Secondary Antibodies (26)

    HRP-conjugated Goat anti-Rabbit IgG (H+L)
    AS014

    WBDBIHC-PELISA

    HRP-conjugated Goat anti-Rabbit IgG (H+L)

    FITC-conjugated Goat anti-Rabbit IgG (H+L)
    AS011

    IF/ICCFC

    FITC-conjugated Goat anti-Rabbit IgG (H+L)

    Cy3-conjugated Goat anti-Rabbit IgG (H+L)
    AS007

    IF/ICCFC

    Cy3-conjugated Goat anti-Rabbit IgG (H+L)

    ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
    AS053

    IF/ICCFC

    ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

    HRP-conjugated Mouse anti-Rabbit IgG Light Chain
    AS061

    WBIP

    HRP-conjugated Mouse anti-Rabbit IgG Light Chain

    HRP-conjugated Goat anti-Rabbit IgG Heavy Chain
    AS063

    WB

    HRP-conjugated Goat anti-Rabbit IgG Heavy Chain

    ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)
    AS039

    IF/ICCFC

    ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)

    Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)
    AS040

    IHC-PIF/ICCFC

    Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)

    ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)
    AS060

    IF/ICCFC

    ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)

    Goat Anti-Rabbit IgG(H+L)
    AS070

    WBELISACUT&Tag

    Goat Anti-Rabbit IgG(H+L)

    HRP-conjugated Donkey anti-Rabbit IgG (H+L)
    AS038

    WBIHC-P

    HRP-conjugated Donkey anti-Rabbit IgG (H+L)

    ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)
    AS035

    IF/ICCFC

    ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)

    ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
    AS073

    IF/ICCFC

    ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

    ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)
    AS058

    IF/ICCFC

    ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)

    FITC-conjugated Donkey anti-Rabbit IgG (H+L)
    AS042

    IF/ICCFC

    FITC-conjugated Donkey anti-Rabbit IgG (H+L)

    ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)
    AS056

    IF/ICCFC

    ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)

    AP-conjugated Goat anti-Rabbit IgG (H+L)
    AS009

    WB

    AP-conjugated Goat anti-Rabbit IgG (H+L)

    HRP-conjugated Mouse anti-Rabbit IgG (H+L)
    AS079

    WB

    HRP-conjugated Mouse anti-Rabbit IgG (H+L)

    HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb
    AS126

    IPELISA

    HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb

    PE Mouse anti-Rabbit IgG, light chain specific mAb
    AS129

    FC

    PE Mouse anti-Rabbit IgG, light chain specific mAb

    HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb
    AS122

    WBELISA

    HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb

    Rabbit Anti-Chicken IgY (H+L)
    AS090

    WB

    Rabbit Anti-Chicken IgY (H+L)

    Rabbit Anti-Chicken IgY Fab
    AS088

    WB

    Rabbit Anti-Chicken IgY Fab

    FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific
    AS083

    IF/ICCFC

    FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific

    Rabbit Anti-Chicken IgY (H+L)
    AS082

    WB

    Rabbit Anti-Chicken IgY (H+L)

    ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)
    AS072

    IF/ICCFC

    ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)