제품 > 항체 > 다중클론항체(pAb)

SOX30 Rabbit pAb (A11759)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using SOX30 Rabbit pAb (A11759) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

Overview

Product name	SOX30 Rabbit pAb
Catalog No.	A11759
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Enables DNA-binding transcription activator activity, RNA polymerase II-specific and DNA-binding transcription repressor activity, RNA polymerase II-specific. Involved in negative regulation of transcription by RNA polymerase II; positive regulation of transcription by RNA polymerase II; and proacrosomal vesicle fusion. Located in chromocenter and nucleus. Is expressed in several structures, including brain; genitourinary system; gut; heart; and lung. Orthologous to human SOX30 (SRY-box transcription factor 30).

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of mouse SOX30 (NP_775560.1).
Sequence	MERARPEPPPPPPPPPRQPPRPTPPRPLRPAPPAQPVEAATFRAAAAERSQSPSAQATAAMAAVASSCGEAAAAGAQAAGTRRLLQVKPEQVLLLPPGGPGVPPAPDEGAAAAAAAAAAAASSAQARLLQLRPELLLLPPQSAADGGPCR
Gene ID
Swiss Prot
Synonyms	SOX30
Calculated MW	84kDa
Observed MW	110kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	U-87MG, BxPC-3, SGC-7901, Mouse pancreas, Mouse stomach, Rat brain
Cellular location	Nucleus
Customer validation	IHC(Mus musculus) WB(Mus musculus) IF(Mus musculus) IP(Mus musculus)