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SRSF3 Rabbit mAb (A9054)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - SRSF3 Rabbit mAb (A9054)

Western blot analysis of various lysates using SRSF3 Rabbit mAb (A9054) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - SRSF3 Rabbit mAb (A9054)

Immunohistochemistry analysis of SRSF3 in paraffin-embedded mouse colon tissue using SRSF3 Rabbit mAb (A9054) at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - SRSF3 Rabbit mAb (A9054)

Immunohistochemistry analysis of SRSF3 in paraffin-embedded rat kidney tissue using SRSF3 Rabbit mAb (A9054) at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - SRSF3 Rabbit mAb (A9054)

Immunohistochemistry analysis of SRSF3 in paraffin-embedded rat spleen tissue using SRSF3 Rabbit mAb (A9054) at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Overview

Product nameSRSF3 Rabbit mAb
Catalog No.A9054
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1394
The protein encoded by this gene is a member of the serine/arginine (SR)-rich family of pre-mRNA splicing factors, which constitute part of the spliceosome. Each of these factors contains an RNA recognition motif (RRM) for binding RNA and an RS domain for binding other proteins. The RS domain is rich in serine and arginine residues and facilitates interaction between different SR splicing factors. In addition to being critical for mRNA splicing, the SR proteins have also been shown to be involved in mRNA export from the nucleus and in translation. Two transcript variants, one protein-coding and the other non-coding, have been found for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human SRSF3 (P84103).
SequenceMHRDSCPLDCKVYVGNLGNNGNKTELERAFGYYGPLRSVWVARNPPGFAFVEFEDPRDAADAVRELDGRTLCGCRVRVELSNGEKRSRNRGPPPSWGRRP
Gene ID
Swiss Prot
SynonymsSFRS3; SRp20; SRSF3
Calculated MW19kDa
Observed MW20kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesK-562, U-251MG, Mouse brain, Rat spleen
Cellular locationCytoplasm, Nucleus
Customer validation

WB(Homo sapiens)

Documents

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ABclonal:Western blot - SRSF3 Rabbit mAb (A9054)}

Western blot - SRSF3 Rabbit mAb (A9054)

Western blot analysis of various lysates using SRSF3 Rabbit mAb (A9054) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - SRSF3 Rabbit mAb (A9054)}

Immunohistochemistry - SRSF3 Rabbit mAb (A9054)

Immunohistochemistry analysis of SRSF3 in paraffin-embedded mouse colon tissue using SRSF3 Rabbit mAb (A9054) at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - SRSF3 Rabbit mAb (A9054)}

Immunohistochemistry - SRSF3 Rabbit mAb (A9054)

Immunohistochemistry analysis of SRSF3 in paraffin-embedded rat kidney tissue using SRSF3 Rabbit mAb (A9054) at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - SRSF3 Rabbit mAb (A9054)}

Immunohistochemistry - SRSF3 Rabbit mAb (A9054)

Immunohistochemistry analysis of SRSF3 in paraffin-embedded rat spleen tissue using SRSF3 Rabbit mAb (A9054) at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)