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Somatostatin Receptor 2 (SSTR2) Rabbit mAb (A21970)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Somatostatin Receptor 2 (SSTR2) Rabbit mAb (A21970)

Western blot analysis of various lysates, using SomatostatinReceptor2(SSTR2) antibody (A21970) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Western blot - Somatostatin Receptor 2 (SSTR2) Rabbit mAb (A21970)

Western blot analysis of C6, using SomatostatinReceptor2(SSTR2) antibody (A21970) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product nameSomatostatin Receptor 2 (SSTR2) Rabbit mAb
Catalog No.A21970
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC54043
Somatostatin acts at many sites to inhibit the release of many hormones and other secretory proteins. The biologic effects of somatostatin are probably mediated by a family of G protein-coupled receptors that are expressed in a tissue-specific manner. SSTR2 is a member of the superfamily of receptors having seven transmembrane segments and is expressed in highest levels in cerebrum and kidney.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 302-369 of human Somatostatin Receptor 2 (SSTR2) (NP_001041.1).
SequenceYANSCANPILYAFLSDNFKKSFQNVLCLVKVSGTDDGERSDSKQDKSRLNETTETQRTLLNGDLQTSI
Gene ID
Swiss Prot
SynonymsSST2
Calculated MW41kDa
Observed MW87kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesSH-SY5Y, Mouse thymus, Neuro-2a, C6
Cellular locationCell membrane, Cytoplasm, Multi-pass membrane protein

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ABclonal:Western blot - Somatostatin Receptor 2 (SSTR2) Rabbit mAb (A21970)}

Western blot - Somatostatin Receptor 2 (SSTR2) Rabbit mAb (A21970)

Western blot analysis of various lysates, using SomatostatinReceptor2(SSTR2) antibody (A21970) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - Somatostatin Receptor 2 (SSTR2) Rabbit mAb (A21970)}

Western blot - Somatostatin Receptor 2 (SSTR2) Rabbit mAb (A21970)

Western blot analysis of C6, using SomatostatinReceptor2(SSTR2) antibody (A21970) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

* For research use only. Not for therapeutic or diagnostic purposes.

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