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Somatostatin Receptor 2 (SSTR2) Rabbit pAb (A15101)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Somatostatin Receptor 2 (SSTR2) Rabbit pAb (A15101)

Western blot analysis of various lysates using Somatostatin Receptor 2 (SSTR2) Rabbit pAb (A15101) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

ABclonal:Immunofluorescence - Somatostatin Receptor 2 (SSTR2) Rabbit pAb (A15101)

Immunofluorescence analysis of C6 cells using Somatostatin Receptor 2 (SSTR2) Rabbit pAb (A15101) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Somatostatin Receptor 2 (SSTR2) Rabbit pAb (A15101)

Immunofluorescence analysis of L929 cells using Somatostatin Receptor 2 (SSTR2) Rabbit pAb (A15101) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Somatostatin Receptor 2 (SSTR2) Rabbit pAb (A15101)

Immunofluorescence analysis of U-2 OS cells using Somatostatin Receptor 2 (SSTR2) Rabbit pAb (A15101) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameSomatostatin Receptor 2 (SSTR2) Rabbit pAb
Catalog No.A15101
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Somatostatin acts at many sites to inhibit the release of many hormones and other secretory proteins. The biologic effects of somatostatin are probably mediated by a family of G protein-coupled receptors that are expressed in a tissue-specific manner. SSTR2 is a member of the superfamily of receptors having seven transmembrane segments and is expressed in highest levels in cerebrum and kidney.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 270-369 of human Somatostatin Receptor 2 (SSTR2) (NP_001041.1).
SequenceLPFYIFNVSSVSMAISPTPALKGMFDFVVVLTYANSCANPILYAFLSDNFKKSFQNVLCLVKVSGTDDGERSDSKQDKSRLNETTETQRTLLNGDLQTSI
Gene ID
Swiss Prot
SynonymsSST2; Somatostatin Receptor 2 (SSTR2)
Calculated MW41kDa
Observed MW56kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesmouse kidney, mouse liver
Cellular locationCell membrane, Cytoplasm, Multi-pass membrane protein

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ABclonal:Western blot - Somatostatin Receptor 2 (SSTR2) Rabbit pAb (A15101)}

Western blot - Somatostatin Receptor 2 (SSTR2) Rabbit pAb (A15101)

Western blot analysis of various lysates using Somatostatin Receptor 2 (SSTR2) Rabbit pAb (A15101) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.
ABclonal:Immunofluorescence - Somatostatin Receptor 2 (SSTR2) Rabbit pAb (A15101)}

Immunofluorescence - Somatostatin Receptor 2 (SSTR2) Rabbit pAb (A15101)

Immunofluorescence analysis of C6 cells using Somatostatin Receptor 2 (SSTR2) Rabbit pAb (A15101) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Somatostatin Receptor 2 (SSTR2) Rabbit pAb (A15101)}

Immunofluorescence - Somatostatin Receptor 2 (SSTR2) Rabbit pAb (A15101)

Immunofluorescence analysis of L929 cells using Somatostatin Receptor 2 (SSTR2) Rabbit pAb (A15101) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Somatostatin Receptor 2 (SSTR2) Rabbit pAb (A15101)}

Immunofluorescence - Somatostatin Receptor 2 (SSTR2) Rabbit pAb (A15101)

Immunofluorescence analysis of U-2 OS cells using Somatostatin Receptor 2 (SSTR2) Rabbit pAb (A15101) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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