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StAR Rabbit mAb (A22166)

Publications (3) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - StAR Rabbit mAb (A22166)

Western blot analysis of various lysates, using StAR Rabbit mAb (A22166) at1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - StAR Rabbit mAb (A22166)

Western blot analysis of lysates from K-562 cells, using StAR Rabbit mAb (A22166) at1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - StAR Rabbit mAb (A22166)

Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using StAR Rabbit mAb (A22166) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - StAR Rabbit mAb (A22166)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using StAR Rabbit mAb (A22166) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - StAR Rabbit mAb (A22166)

Confocal imaging of paraffin-embedded Rat adrenal gland using StAR Rabbit mAb (A22166, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citRate buffer (pH 6.0) prior to IF staining.

ABclonal:Immunofluorescence - StAR Rabbit mAb (A22166)

Confocal imaging of paraffin-embedded Mouse adrenal gland using StAR Rabbit mAb (A22166, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citRate buffer (pH 6.0) prior to IF staining.

ABclonal:Immunofluorescence - StAR Rabbit mAb (A22166)

Confocal imaging of paraffin-embedded Human adrenal gland using StAR Rabbit mAb (A22166, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citRate buffer (pH 6.0) prior to IF staining.

Overview

Product nameStAR Rabbit mAb
Catalog No.A22166
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC55812
The protein encoded by this gene plays a key role in the acute regulation of steroid hormone synthesis by enhancing the conversion of cholesterol into pregnenolone. This protein permits the cleavage of cholesterol into pregnenolone by mediating the transport of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane. Mutations in this gene are a cause of congenital lipoid adrenal hyperplasia (CLAH), also called lipoid CAH. A pseudogene of this gene is located on chromosome 13.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 100-200 of human StAR (NP_000340.2).
SequenceSQQDNGDKVMSKVVPDVGKVFRLEVVVDQPMERLYEELVERMEAMGEWNPNVKEIKVLQKIGKDTFITHELAAEAAGNLVGPRDFVSVRCAKRRGSTCVLA
Gene ID
Swiss Prot
SynonymsSTARD1; StAR
Calculated MW32kDa
Observed MW28kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2000 - 1:8000
  • IHC-P 1:200 - 1:800
  • IF/ICC 1:200 - 1:2000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesK-562, Mouse testis, Rat testis
Cellular locationMitochondrion.
Customer validation

WB(Rattus norvegicus)

IF(Gallus gallus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - StAR Rabbit mAb (A22166)}

Western blot - StAR Rabbit mAb (A22166)

Western blot analysis of various lysates, using StAR Rabbit mAb (A22166) at1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - StAR Rabbit mAb (A22166)}

Western blot - StAR Rabbit mAb (A22166)

Western blot analysis of lysates from K-562 cells, using StAR Rabbit mAb (A22166) at1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - StAR Rabbit mAb (A22166)}

Immunohistochemistry - StAR Rabbit mAb (A22166)

Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using StAR Rabbit mAb (A22166) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - StAR Rabbit mAb (A22166)}

Immunohistochemistry - StAR Rabbit mAb (A22166)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using StAR Rabbit mAb (A22166) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - StAR Rabbit mAb (A22166)}

Immunofluorescence - StAR Rabbit mAb (A22166)

Confocal imaging of paraffin-embedded Rat adrenal gland using StAR Rabbit mAb (A22166, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citRate buffer (pH 6.0) prior to IF staining.
ABclonal:Immunofluorescence - StAR Rabbit mAb (A22166)}

Immunofluorescence - StAR Rabbit mAb (A22166)

Confocal imaging of paraffin-embedded Mouse adrenal gland using StAR Rabbit mAb (A22166, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citRate buffer (pH 6.0) prior to IF staining.
ABclonal:Immunofluorescence - StAR Rabbit mAb (A22166)}

Immunofluorescence - StAR Rabbit mAb (A22166)

Confocal imaging of paraffin-embedded Human adrenal gland using StAR Rabbit mAb (A22166, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citRate buffer (pH 6.0) prior to IF staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (2)

Secondary Antibodies (26)