Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat
Western blot analysis of extracts of various cell lines, using StAR antibody (A16432) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
Confocal immunofluorescence analysis of mouse adrenal gland using StAR Polyclonal Antibody (A16432) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of Human adrenal gland cells using StAR Rabbit pAb (A16432) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse adrenal gland cells using StAR Rabbit pAb (A16432) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
| Product name | StAR Rabbit pAb |
|---|---|
| Catalog No. | A16432 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 64-285 of human StAR (NP_000340.2). |
|---|---|
| Sequence | EETLYSDQELAYLQQGEEAMQKALGILSNQEGWKKESQQDNGDKVMSKVVPDVGKVFRLEVVVDQPMERLYEELVERMEAMGEWNPNVKEIKVLQKIGKDTFITHELAAEAAGNLVGPRDFVSVRCAKRRGSTCVLAGMATDFGNMPEQKGVIRAEHGPTCMVLHPLAGSPSKTKLTWLLSIDLKGWLPKSIINQVLSQTQVDFANHLRKRLESHPASEARC |
| Gene ID | |
| Swiss Prot | |
| Synonyms | STARD1 |
| Calculated MW | 32kDa |
| Observed MW | 28kDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Key application | Western blotting Immunofluorescence |
| Positive samples | Mouse testis, Rat testis |
| Cellular location | Mitochondrion |
| Customer validation | WB(Capra hircus, Mus musculus, Tianfu broilers , Rattus norvegicus, Danio rerio, Mesocricetus auratus, Gallus gallus, Homo sapiens) IHC(Mus musculus, Homo sapiens, Rattus norvegicus) IF(Labeo bata, Rattus norvegicus, Mus musculus, Gallus gallus) IHC(Rattus norvegicus) WB(Rattus norvegicus, Gallus gallus) IHC(Rattus norvegicus) IF(Rattus norvegicus) RT-qPCR(Rattus norvegicus) RT-qPCR(Mus musculus) RT-PCR(Gallus gallus) |
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* For research use only. Not for therapeutic or diagnostic purposes.
Publishing research using A16432? Please let us know so that we can cite the reference in this datasheet.
