Review (1)Publications (12) Datasheet
Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse
Western blot analysis of various lysates using StAR Rabbit pAb (A1035) at 1:400 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Immunoprecipitation analysis of 300 μg extracts of K-562 cells using 3 μg StAR antibody (A1035). Western blot was performed from the immunoprecipitate using StAR antibody (A1035) at a dilution of 1:1000.
| Product name | StAR Rabbit pAb |
|---|---|
| Catalog No. | A1035 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 64-285 of human StAR (NP_000340.2). |
|---|---|
| Sequence | EETLYSDQELAYLQQGEEAMQKALGILSNQEGWKKESQQDNGDKVMSKVVPDVGKVFRLEVVVDQPMERLYEELVERMEAMGEWNPNVKEIKVLQKIGKDTFITHELAAEAAGNLVGPRDFVSVRCAKRRGSTCVLAGMATDFGNMPEQKGVIRAEHGPTCMVLHPLAGSPSKTKLTWLLSIDLKGWLPKSIINQVLSQTQVDFANHLRKRLESHPASEARC |
| Gene ID | |
| Swiss Prot | |
| Synonyms | STARD1; StAR |
| Calculated MW | 32kDa |
| Observed MW | 28kDa |
| Reactivity | Human, Mouse |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3. |
| Key application | Western blotting Immunoprecipitation |
| Positive samples | HEL, K-562 |
| Cellular location | Mitochondrion. |
| Customer validation | (Human breast cancer) WB(Sus scrofa, Mus musculus, Gallus gallus, Rattus norvegicus, Sus domesticus, Capra hircus, Other) IHC(Mus musculus) IHC(Capra hircus) mIHC(Capra hircus) |
To download a Certificate of Compliance, please enter your Lot number below:
Lot number
* For research use only. Not for therapeutic or diagnostic purposes.
Please submit reviews to your technical sales specialist directly. Alternatively, email us at info@abclonal.co.kr
Publishing research using A1035? Please let us know so that we can cite the reference in this datasheet.
