제품 > 항체 > 다중클론항체(pAb)

TASL Rabbit pAb (A20486)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - TASL Rabbit pAb (A20486)

Western blot analysis of lysates from Mouse lung, using TASL Rabbit pAb (A20486) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - TASL Rabbit pAb (A20486)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using TASL Rabbit pAb (A20486) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Overview

Product nameTASL Rabbit pAb
Catalog No.A20486
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Involved in positive regulation of innate immune response; positive regulation of toll-like receptor signaling pathway; and regulation of lysosomal lumen pH. Located in endolysosome membrane.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 96-196 of human TASL (NP_079435.1).
SequenceNLAAVEICRDASRETYLVPSSCKSICKNYNDLQIAGGQVMAINSVTTDFPSESSFEYGPLLKSSEIPLPMEDSISTQPSDFPQKPIQRYSSYWRITSIKEK
Gene ID
Swiss Prot
SynonymsCXorf21; TASL
Calculated MW34kDa
Observed MW35kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesMouse lung
Cellular locationnucleoplasm.
Customer validation

IHC(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - TASL Rabbit pAb (A20486)}

    Western blot - TASL Rabbit pAb (A20486)

    Western blot analysis of lysates from Mouse lung, using TASL Rabbit pAb (A20486) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Immunohistochemistry - TASL Rabbit pAb (A20486)}

    Immunohistochemistry - TASL Rabbit pAb (A20486)

    Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using TASL Rabbit pAb (A20486) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A20486? Please let us know so that we can cite the reference in this datasheet.

    Secondary Antibodies (26)