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TAZ Rabbit PolymAb® (A26425PM)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - TAZ Rabbit PolymAb® (A26425PM)

Western blot analysis of lysates from HeLa cells using TAZ Rabbit PolymAb® (A26425PM)at 1:3000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.

ABclonal:Immunohistochemistry - TAZ Rabbit PolymAb® (A26425PM)

Immunohistochemistry analysis of paraffin-embedded Rat lung tissue using TAZ Rabbit PolymAb® (A26425PM) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - TAZ Rabbit PolymAb® (A26425PM)

Immunohistochemistry analysis of paraffin-embedded Mouse lung tissue using TAZ Rabbit PolymAb® (A26425PM) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - TAZ Rabbit PolymAb® (A26425PM)

Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using TAZ Rabbit PolymAb® (A26425PM) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - TAZ Rabbit PolymAb® (A26425PM)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using TAZ Rabbit PolymAb® (A26425PM) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - TAZ Rabbit PolymAb® (A26425PM)

Immunohistochemistry analysis of paraffin-embedded Human colon tissue using TAZ Rabbit PolymAb® (A26425PM) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

Overview

Product nameTAZ Rabbit PolymAb®
Catalog No.A26425PM
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables transcription coactivator activity. Involved in several processes, including hippo signaling; positive regulation of cell differentiation; and regulation of signal transduction. Located in cytosol and nuclear body.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human TAZ (NP_056287.1)
SequenceMNPASAPPPLPPPGQQVIHVTQDLDTDLEALFNSVMNPKPSSWRKKILPESFFKEPDSGSHSRQSSTDSSGGHPGPRLAGGAQHVRSHSSPASLQLGTGAGAAGSPAQQHAHLRQQSYDVTDELPLPPGWEMTFTATGQRYFLNHIEKITTWQDPRKAMNQPLNHMNLHPAVSSTPVPQRSMAVSQPNLVMNHQHQQQMAPSTLSQQNHPTQNPPAGLMSMPNALTTQQQQQQKLRLQRIQMERERIRMRQEELMRQEAALCRQLPMEAE
Gene ID
Swiss Prot
SynonymsTAZ
Calculated MW44kDa
Observed MW60kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1500 - 1:9000
  • IHC-P 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHeLa
Cellular locationCytoplasm, Nucleus.

Documents

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ABclonal:Western blot - TAZ Rabbit PolymAb® (A26425PM)}

Western blot - TAZ Rabbit PolymAb® (A26425PM)

Western blot analysis of lysates from HeLa cells using TAZ Rabbit PolymAb® (A26425PM)at 1:3000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.
ABclonal:Immunohistochemistry - TAZ Rabbit PolymAb® (A26425PM)}

Immunohistochemistry - TAZ Rabbit PolymAb® (A26425PM)

Immunohistochemistry analysis of paraffin-embedded Rat lung tissue using TAZ Rabbit PolymAb® (A26425PM) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - TAZ Rabbit PolymAb® (A26425PM)}

Immunohistochemistry - TAZ Rabbit PolymAb® (A26425PM)

Immunohistochemistry analysis of paraffin-embedded Mouse lung tissue using TAZ Rabbit PolymAb® (A26425PM) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - TAZ Rabbit PolymAb® (A26425PM)}

Immunohistochemistry - TAZ Rabbit PolymAb® (A26425PM)

Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using TAZ Rabbit PolymAb® (A26425PM) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - TAZ Rabbit PolymAb® (A26425PM)}

Immunohistochemistry - TAZ Rabbit PolymAb® (A26425PM)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using TAZ Rabbit PolymAb® (A26425PM) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - TAZ Rabbit PolymAb® (A26425PM)}

Immunohistochemistry - TAZ Rabbit PolymAb® (A26425PM)

Immunohistochemistry analysis of paraffin-embedded Human colon tissue using TAZ Rabbit PolymAb® (A26425PM) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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