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TIMM50 Rabbit mAb (A1536)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - TIMM50 Rabbit mAb (A1536)

Western blot analysis of various lysates using TIMM50 Rabbit mAb (A1536) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - TIMM50 Rabbit mAb (A1536)

Western blot analysis of various lysates using TIMM50 Rabbit mAb (A1536) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - TIMM50 Rabbit mAb (A1536)

Immunohistochemistry analysis of TIMM50 in paraffin-embedded mouse kidney tissue using TIMM50 Rabbit mAb (A1536) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - TIMM50 Rabbit mAb (A1536)

Immunohistochemistry analysis of TIMM50 in paraffin-embedded human liver tissue using TIMM50 Rabbit mAb (A1536) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - TIMM50 Rabbit mAb (A1536)

Immunohistochemistry analysis of TIMM50 in paraffin-embedded rat kidney tissue using TIMM50 Rabbit mAb (A1536) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunofluorescence - TIMM50 Rabbit mAb (A1536)

Confocal imaging of NIH/3T3 cells using TIMM50 Rabbit mAb (A1536, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunofluorescence - TIMM50 Rabbit mAb (A1536)

Confocal imaging of paraffin-embedded Mouse kidney tissue using TIMM50 Rabbit mAb (A1536, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

ABclonal:Immunofluorescence - TIMM50 Rabbit mAb (A1536)

Confocal imaging of paraffin-embedded Human kidney tissue using TIMM50 Rabbit mAb (A1536, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Overview

Product nameTIMM50 Rabbit mAb
Catalog No.A1536
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1883
This gene encodes a subunit of the TIM23 inner mitochondrial membrane translocase complex. The encoded protein functions as the receptor subunit that recognizes the mitochondrial targeting signal, or presequence, on protein cargo that is destined for the mitochondrial inner membrane and matrix. This protein may also play a role in maintaining the membrane permeability barrier, and knockdown of this gene in human cells results in the release of cytochrome c and apoptosis.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human TIMM50 (Q3ZCQ8).
SequenceMAASAAVFSRLRSGLRLGSRGLCTRLATPPRRAPDQAAEIGSRGSTKAQGPQQQPGSEGPSYAKKVALWLAGLLGAGGTVSVVYIFGNNPVDENGAKIPD
Gene ID
Swiss Prot
SynonymsMGCA9; TIM50; TIM50L; TIMM50
Calculated MW40kDa
Observed MW38kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples293T, HepG2, Mouse liver, Mouse brain
Cellular locationMitochondrion inner membrane, Nucleus speckle, Single-pass membrane protein

Documents

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ABclonal:Western blot - TIMM50 Rabbit mAb (A1536)}

Western blot - TIMM50 Rabbit mAb (A1536)

Western blot analysis of various lysates using TIMM50 Rabbit mAb (A1536) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - TIMM50 Rabbit mAb (A1536)}

Western blot - TIMM50 Rabbit mAb (A1536)

Western blot analysis of various lysates using TIMM50 Rabbit mAb (A1536) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry - TIMM50 Rabbit mAb (A1536)}

Immunohistochemistry - TIMM50 Rabbit mAb (A1536)

Immunohistochemistry analysis of TIMM50 in paraffin-embedded mouse kidney tissue using TIMM50 Rabbit mAb (A1536) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - TIMM50 Rabbit mAb (A1536)}

Immunohistochemistry - TIMM50 Rabbit mAb (A1536)

Immunohistochemistry analysis of TIMM50 in paraffin-embedded human liver tissue using TIMM50 Rabbit mAb (A1536) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - TIMM50 Rabbit mAb (A1536)}

Immunohistochemistry - TIMM50 Rabbit mAb (A1536)

Immunohistochemistry analysis of TIMM50 in paraffin-embedded rat kidney tissue using TIMM50 Rabbit mAb (A1536) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunofluorescence - TIMM50 Rabbit mAb (A1536)}

Immunofluorescence - TIMM50 Rabbit mAb (A1536)

Confocal imaging of NIH/3T3 cells using TIMM50 Rabbit mAb (A1536, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
ABclonal:Immunofluorescence - TIMM50 Rabbit mAb (A1536)}

Immunofluorescence - TIMM50 Rabbit mAb (A1536)

Confocal imaging of paraffin-embedded Mouse kidney tissue using TIMM50 Rabbit mAb (A1536, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
ABclonal:Immunofluorescence - TIMM50 Rabbit mAb (A1536)}

Immunofluorescence - TIMM50 Rabbit mAb (A1536)

Confocal imaging of paraffin-embedded Human kidney tissue using TIMM50 Rabbit mAb (A1536, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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