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TIRAP Rabbit mAb (A9663)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - TIRAP Rabbit mAb (A9663)

Western blot analysis of various lysates using TIRAP Rabbit mAb (A9663) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - TIRAP Rabbit mAb (A9663)

Immunohistochemistry analysis of paraffin-embedded Rat lung using TIRAP Rabbit mAb (A9663) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - TIRAP Rabbit mAb (A9663)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using TIRAP Rabbit mAb (A9663) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Overview

Product nameTIRAP Rabbit mAb
Catalog No.A9663
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1686
The innate immune system recognizes microbial pathogens through Toll-like receptors (TLRs), which identify pathogen-associated molecular patterns. Different TLRs recognize different pathogen-associated molecular patterns and all TLRs have a Toll-interleukin 1 receptor (TIR) domain, which is responsible for signal transduction. The protein encoded by this gene is a TIR adaptor protein involved in the TLR4 signaling pathway of the immune system. It activates NF-kappa-B, MAPK1, MAPK3 and JNK, which then results in cytokine secretion and the inflammatory response. Alternative splicing of this gene results in several transcript variants; however, not all variants have been fully described.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human TIRAP (P58753).
SequenceMASSTSLPAPGSRPKKPLGKMADWFRQTLLKKPKKRPNSPESTSSDASQPTSQDSPLPPSLSSVTSPSLPPTHASDSGSSRWSKDYDVCVCHSEEDLVAA
Gene ID
Swiss Prot
SynonymsMal; wyatt; BACTS1; MyD88-2; TIRAP
Calculated MW24kDa
Observed MW32kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samples293T, Hep G2, THP-1, Mouse brain, Rat spleen, Rat thymus
Cellular locationCell membrane, Cytoplasm, Membrane
Customer validation

WB(Homo sapiens)

Documents

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ABclonal:Western blot - TIRAP Rabbit mAb (A9663)}

Western blot - TIRAP Rabbit mAb (A9663)

Western blot analysis of various lysates using TIRAP Rabbit mAb (A9663) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - TIRAP Rabbit mAb (A9663)}

Immunohistochemistry - TIRAP Rabbit mAb (A9663)

Immunohistochemistry analysis of paraffin-embedded Rat lung using TIRAP Rabbit mAb (A9663) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - TIRAP Rabbit mAb (A9663)}

Immunohistochemistry - TIRAP Rabbit mAb (A9663)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using TIRAP Rabbit mAb (A9663) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A9663? Please let us know so that we can cite the reference in this datasheet.

항체 (2)

Secondary Antibodies (26)