제품 > 항체 > 다중클론항체(pAb)

TNKS Rabbit pAb (A17027)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of various lysates using TNKS Rabbit pAb (A17027) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Overview

Product name	TNKS Rabbit pAb
Catalog No.	A17027
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Enables histone binding activity; pentosyltransferase activity; and zinc ion binding activity. Involved in several processes, including negative regulation of maintenance of mitotic sister chromatid cohesion, telomeric; protein ADP-ribosylation; and regulation of nucleobase-containing compound metabolic process. Acts upstream of or within peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; and protein ADP-ribosylation. Located in several cellular components, including chromosome, telomeric region; mitotic spindle pole; and nucleus.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 1050-1150 of human TNKS (NP_003738.2).
Sequence	EQITLDVLADMGHEELKEIGINAYGHRHKLIKGVERLLGGQQGTNPYLTFHCVNQGTILLDLAPEDKEYQSVEEEMQSTIREHRDGGNAGGIFNRYNVIRI
Gene ID
Swiss Prot
Synonyms	TIN1; ARTD5; PARPL; TINF1; TNKS1; pART5; PARP5A; PARP-5a; TNKS
Calculated MW	142kDa
Observed MW	179kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	Raji, U-251MG
Cellular location	cytoplasm, cytosol, Golgi apparatus, mitotic spindle pole, nuclear body, nuclear membrane, nuclear pore, nucleoplasm, nucleus