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TRAP1 Rabbit pAb (A2748)

Review (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - TRAP1 Rabbit pAb (A2748)

Western blot analysis of various lysates, using TRAP1 Rabbit pAb (A2748) at 1:800 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Western blot - TRAP1 Rabbit pAb (A2748)

Western blot analysis of lysates from NIH/3T3 cells, using TRAP1 Rabbit pAb (A2748) at 1:800 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - TRAP1 Rabbit pAb (A2748)

Immunohistochemistry analysis of paraffin-embedded Rat heart using TRAP1 Rabbit pAb (A2748) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - TRAP1 Rabbit pAb (A2748)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using TRAP1 Rabbit pAb (A2748) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - TRAP1 Rabbit pAb (A2748)

Immunohistochemistry analysis of paraffin-embedded Mouse heart using TRAP1 Rabbit pAb (A2748) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - TRAP1 Rabbit pAb (A2748)

Immunofluorescence analysis of HCT 116 cells using TRAP1 Rabbit pAb (A2748) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - TRAP1 Rabbit pAb (A2748)

Immunofluorescence analysis of HeLa cells using TRAP1 Rabbit pAb (A2748) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - TRAP1 Rabbit pAb (A2748)

Immunofluorescence analysis of HepG2 cells using TRAP1 Rabbit pAb (A2748) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for TRAP1 Rabbit pAb(A2748)

Overview

Product nameTRAP1 Rabbit pAb
Catalog No.A2748
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a mitochondrial chaperone protein that is member of the heat shock protein 90 (HSP90) family. The encoded protein has ATPase activity and interacts with tumor necrosis factor type I. This protein may function in regulating cellular stress responses. Alternate splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 445-704 of human TRAP1 (NP_057376.2).
SequenceGLFMREGIVTATEQEVKEDIAKLLRYESSALPSGQLTSLSEYASRMRAGTRNIYYLCAPNRHLAEHSPYYEAMKKKDTEVLFCFEQFDELTLLHLREFDKKKLISVETDIVVDHYKEEKFEDRSPAAECLSEKETEELMAWMRNVLGSRVTNVKVTLRLDTHPAMVTVLEMGAARHFLRMQQLAKTQEERAQLLQPTLEINPRHALIKKLNQLRASEPGLAQLLVDQIYENAMIAAGLVDDPRAMVGRLNELLVKALERH
Gene ID
Swiss Prot
SynonymsHSP75; HSP 75; HSP90L; TRAP-1; TRAP1
Calculated MW80kDa
Observed MW71kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, HCT 116, NIH/3T3
Cellular locationMitochondrion, Mitochondrion inner membrane, Mitochondrion matrix.
Customer validation

(Huh7.5.1,HepG2)

Documents

Certificate of Compliance

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ABclonal:Western blot - TRAP1 Rabbit pAb (A2748)}

Western blot - TRAP1 Rabbit pAb (A2748)

Western blot analysis of various lysates, using TRAP1 Rabbit pAb (A2748) at 1:800 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Western blot - TRAP1 Rabbit pAb (A2748)}

Western blot - TRAP1 Rabbit pAb (A2748)

Western blot analysis of lysates from NIH/3T3 cells, using TRAP1 Rabbit pAb (A2748) at 1:800 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - TRAP1 Rabbit pAb (A2748)}

Immunohistochemistry - TRAP1 Rabbit pAb (A2748)

Immunohistochemistry analysis of paraffin-embedded Rat heart using TRAP1 Rabbit pAb (A2748) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - TRAP1 Rabbit pAb (A2748)}

Immunohistochemistry - TRAP1 Rabbit pAb (A2748)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using TRAP1 Rabbit pAb (A2748) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - TRAP1 Rabbit pAb (A2748)}

Immunohistochemistry - TRAP1 Rabbit pAb (A2748)

Immunohistochemistry analysis of paraffin-embedded Mouse heart using TRAP1 Rabbit pAb (A2748) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunofluorescence - TRAP1 Rabbit pAb (A2748)}

Immunofluorescence - TRAP1 Rabbit pAb (A2748)

Immunofluorescence analysis of HCT 116 cells using TRAP1 Rabbit pAb (A2748) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - TRAP1 Rabbit pAb (A2748)}

Immunofluorescence - TRAP1 Rabbit pAb (A2748)

Immunofluorescence analysis of HeLa cells using TRAP1 Rabbit pAb (A2748) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - TRAP1 Rabbit pAb (A2748)}

Immunofluorescence - TRAP1 Rabbit pAb (A2748)

Immunofluorescence analysis of HepG2 cells using TRAP1 Rabbit pAb (A2748) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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