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TRMT61A Rabbit mAb (A25353)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - TRMT61A Rabbit mAb (A25353)

Western blot analysis of various lysates using TRMT61A Rabbit mAb (A25353) at 1:3000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - TRMT61A Rabbit mAb (A25353)

Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using TRMT61A Rabbit mAb (A25353) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - TRMT61A Rabbit mAb (A25353)

Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using TRMT61A Rabbit mAb (A25353) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - TRMT61A Rabbit mAb (A25353)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using TRMT61A Rabbit mAb (A25353) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - TRMT61A Rabbit mAb (A25353)

Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using TRMT61A Rabbit mAb (A25353) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - TRMT61A Rabbit mAb (A25353)

Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using TRMT61A Rabbit mAb (A25353) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - TRMT61A Rabbit mAb (A25353)

Confocal imaging of PC-3 cells using TRMT61A Rabbit mAb (A25353, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunofluorescence - TRMT61A Rabbit mAb (A25353)

Confocal imaging of paraffin-embedded Rat spleen tissue using TRMT61A Rabbit mAb (A25353, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

Overview

Product nameTRMT61A Rabbit mAb
Catalog No.A25353
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC66031
Enables mRNA (adenine-N1-)-methyltransferase activity. Involved in mRNA methylation. Predicted to be located in nucleoplasm. Predicted to be part of tRNA (m1A) methyltransferase complex. Predicted to be active in nucleus.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-289 of human TRMT61A (NP_689520.2).
SequenceMSFVAYEELIKEGDTAILSLGHGAMVAVRVQRGAQTQTRHGVLRHSVDLIGRPFGSKVTCGRGGWVYVLHPTPELWTLNLPHRTQILYSTDIALITMMLELRPGSVVCESGTGSGSVSHAIIRTIAPTGHLHTVEFHQQRAEKAREEFQEHRVGRWVTVRTQDVCRSGFGVSHVADAVFLDIPSPWEAVGHAWDALKVEGGRFCSFSPCIEQVQRTCQALAARGFSELSTLEVLPQVYNVRTVSLPPPDLGTGTDGPAGSDTSPFRSGTPMKEAVGHTGYLTFATKTPG
Gene ID
Swiss Prot
SynonymsGCD14; TRM61; Gcd14p; hTRM61; C14orf172
Calculated MW31kDa
Observed MW34kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:3000 - 1:12000
  • IHC-P 1:200 - 1:800
  • IF/ICC 1:200 - 1:800
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesPC-3, MCF7, A549, Mouse spleen
Cellular locationNucleus.

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - TRMT61A Rabbit mAb (A25353)}

    Western blot - TRMT61A Rabbit mAb (A25353)

    Western blot analysis of various lysates using TRMT61A Rabbit mAb (A25353) at 1:3000 dilution incubated overnight at 4℃.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates / proteins: 25 μg per lane.
    Blocking buffer: 3 % nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Immunohistochemistry - TRMT61A Rabbit mAb (A25353)}

    Immunohistochemistry - TRMT61A Rabbit mAb (A25353)

    Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using TRMT61A Rabbit mAb (A25353) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - TRMT61A Rabbit mAb (A25353)}

    Immunohistochemistry - TRMT61A Rabbit mAb (A25353)

    Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using TRMT61A Rabbit mAb (A25353) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - TRMT61A Rabbit mAb (A25353)}

    Immunohistochemistry - TRMT61A Rabbit mAb (A25353)

    Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using TRMT61A Rabbit mAb (A25353) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - TRMT61A Rabbit mAb (A25353)}

    Immunohistochemistry - TRMT61A Rabbit mAb (A25353)

    Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using TRMT61A Rabbit mAb (A25353) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - TRMT61A Rabbit mAb (A25353)}

    Immunohistochemistry - TRMT61A Rabbit mAb (A25353)

    Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using TRMT61A Rabbit mAb (A25353) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunofluorescence - TRMT61A Rabbit mAb (A25353)}

    Immunofluorescence - TRMT61A Rabbit mAb (A25353)

    Confocal imaging of PC-3 cells using TRMT61A Rabbit mAb (A25353, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
    ABclonal:Immunofluorescence - TRMT61A Rabbit mAb (A25353)}

    Immunofluorescence - TRMT61A Rabbit mAb (A25353)

    Confocal imaging of paraffin-embedded Rat spleen tissue using TRMT61A Rabbit mAb (A25353, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

    * For research use only. Not for therapeutic or diagnostic purposes.

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