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Talin 2 Rabbit mAb (A19810)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Talin 2 Rabbit mAb (A19810)

Western blot analysis of various lysates using Talin 2 Rabbit mAb (A19810) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - Talin 2 Rabbit mAb (A19810)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using Talin 2 Rabbit mAb (A19810) at a dilution of 1:500 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Talin 2 Rabbit mAb (A19810)

Immunohistochemistry analysis of paraffin-embedded Human liver tissue using Talin 2 Rabbit mAb (A19810) at a dilution of 1:500 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Talin 2 Rabbit mAb (A19810)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using Talin 2 Rabbit mAb (A19810) at a dilution of 1:500 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Talin 2 Rabbit mAb (A19810)

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using Talin 2 Rabbit mAb (A19810) at a dilution of 1:500 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - Talin 2 Rabbit mAb (A19810)

Confocal imaging of paraffin-embedded Rat brain tissue using Talin 2 Rabbit mAb (A19810, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

ABclonal:Immunofluorescence - Talin 2 Rabbit mAb (A19810)

Confocal imaging of paraffin-embedded Mouse brain tissue using Talin 2 Rabbit mAb (A19810, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Overview

Product nameTalin 2 Rabbit mAb
Catalog No.A19810
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2335
This gene encodes a protein related to talin 1, a cytoskeletal protein that plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. This protein has a different pattern of expression compared to talin 1 but, like talin 1, is thought to associate with unique transmembrane receptors to form novel linkages between extracellular matrices and the actin cytoskeleton.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 350-450 of human Talin 2 (Q9Y4G6).
SequenceVLQEWPLTTVKRWAASPKSFTLDFGEYQESYYSVQTTEGEQISQLIAGYIDIILKKKQSKDRFGLEGDEESTMLEESVSPKKSTILQQQFNRTGKAEHGSV
Gene ID
Swiss Prot
SynonymsILWEQ; TLN2; Talin-2; Talin 2
Calculated MW272kDa
Observed MW272kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:6000
  • IHC-P 1:500 - 1:2000
  • IF/ICC 1:100 - 1:400
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, NIH/3T3, Mouse testis, Rat testis
Cellular locationactin cytoskeleton, cytoplasm, focal adhesion, plasma membrane.
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - Talin 2 Rabbit mAb (A19810)}

Western blot - Talin 2 Rabbit mAb (A19810)

Western blot analysis of various lysates using Talin 2 Rabbit mAb (A19810) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - Talin 2 Rabbit mAb (A19810)}

Immunohistochemistry - Talin 2 Rabbit mAb (A19810)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using Talin 2 Rabbit mAb (A19810) at a dilution of 1:500 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Talin 2 Rabbit mAb (A19810)}

Immunohistochemistry - Talin 2 Rabbit mAb (A19810)

Immunohistochemistry analysis of paraffin-embedded Human liver tissue using Talin 2 Rabbit mAb (A19810) at a dilution of 1:500 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Talin 2 Rabbit mAb (A19810)}

Immunohistochemistry - Talin 2 Rabbit mAb (A19810)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using Talin 2 Rabbit mAb (A19810) at a dilution of 1:500 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Talin 2 Rabbit mAb (A19810)}

Immunohistochemistry - Talin 2 Rabbit mAb (A19810)

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using Talin 2 Rabbit mAb (A19810) at a dilution of 1:500 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - Talin 2 Rabbit mAb (A19810)}

Immunofluorescence - Talin 2 Rabbit mAb (A19810)

Confocal imaging of paraffin-embedded Rat brain tissue using Talin 2 Rabbit mAb (A19810, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
ABclonal:Immunofluorescence - Talin 2 Rabbit mAb (A19810)}

Immunofluorescence - Talin 2 Rabbit mAb (A19810)

Confocal imaging of paraffin-embedded Mouse brain tissue using Talin 2 Rabbit mAb (A19810, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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