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β-TrCP/BTRC Rabbit mAb (A21951)

Publications (3) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - β-TrCP/BTRC Rabbit mAb (A21951)

Western blot analysis of lysates from C2C12 cells, using β-TrCP/BTRC Rabbit mAb (A21951) at1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - β-TrCP/BTRC Rabbit mAb (A21951)

Western blot analysis of lysates from Rat testis, using β-TrCP/BTRC Rabbit mAb (A21951) at1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - β-TrCP/BTRC Rabbit mAb (A21951)

Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using β-TrCP/BTRC Rabbit mAb (A21951) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - β-TrCP/BTRC Rabbit mAb (A21951)

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using β-TrCP/BTRC Rabbit mAb (A21951) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - β-TrCP/BTRC Rabbit mAb (A21951)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using β-TrCP/BTRC Rabbit mAb (A21951) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.

Overview

Product nameβ-TrCP/BTRC Rabbit mAb
Catalog No.A21951
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC51997
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class; in addition to an F-box, this protein contains multiple WD-40 repeats. The encoded protein mediates degradation of CD4 via its interaction with HIV-1 Vpu. It has also been shown to ubiquitinate phosphorylated NFKBIA (nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha), targeting it for degradation and thus activating nuclear factor kappa-B. Alternatively spliced transcript variants have been described. A related pseudogene exists in chromosome 6.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human β-TrCP/BTRC (NP_378663.1).
SequenceMDPAEAVLQEKALKFMCSMPRSLWLGCSSLADSMPSLRCLYNPGTGALTAFQNSSEREDCNNGEPPRKIIPEKNSLRQTYNSCARLCLNQETVCLASTAM
Gene ID
Swiss Prot
SynonymsFWD1; FBW1A; FBXW1; bTrCP; FBXW1A; bTrCP1; betaTrCP; BETA-TRCP; β-TrCP/BTRC
Calculated MW69kDa
Observed MW62kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHeLa, Hep G2, C2C12, Rat testis
Cellular locationCytoplasm, Nucleus
Customer validation

WB(Homo sapiens, Mus musculus, Gallus gallus)

Documents

Certificate of Compliance

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ABclonal:Western blot - β-TrCP/BTRC Rabbit mAb (A21951)}

Western blot - β-TrCP/BTRC Rabbit mAb (A21951)

Western blot analysis of lysates from C2C12 cells, using β-TrCP/BTRC Rabbit mAb (A21951) at1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - β-TrCP/BTRC Rabbit mAb (A21951)}

Western blot - β-TrCP/BTRC Rabbit mAb (A21951)

Western blot analysis of lysates from Rat testis, using β-TrCP/BTRC Rabbit mAb (A21951) at1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - β-TrCP/BTRC Rabbit mAb (A21951)}

Immunohistochemistry - β-TrCP/BTRC Rabbit mAb (A21951)

Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using β-TrCP/BTRC Rabbit mAb (A21951) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - β-TrCP/BTRC Rabbit mAb (A21951)}

Immunohistochemistry - β-TrCP/BTRC Rabbit mAb (A21951)

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using β-TrCP/BTRC Rabbit mAb (A21951) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - β-TrCP/BTRC Rabbit mAb (A21951)}

Immunohistochemistry - β-TrCP/BTRC Rabbit mAb (A21951)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using β-TrCP/BTRC Rabbit mAb (A21951) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A21951? Please let us know so that we can cite the reference in this datasheet.

항체 (1)

Secondary Antibodies (26)