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β-TrCP/BTRC Rabbit pAb (A1656)

Publications (5) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - β-TrCP/BTRC Rabbit pAb (A1656)

Western blot analysis of lysates from U-251MG cells, using β-TrCP/BTRC Rabbit pAb (A1656) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Immunohistochemistry - β-TrCP/BTRC Rabbit pAb (A1656)

Immunohistochemistry analysis of paraffin-embedded mouse spleen using β-TrCP/BTRC Rabbit pAb (A1656) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - β-TrCP/BTRC Rabbit pAb (A1656)

Immunofluorescence analysis of C6 cells using β-TrCP/BTRC Rabbit pAb (A1656) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameβ-TrCP/BTRC Rabbit pAb
Catalog No.A1656
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class; in addition to an F-box, this protein contains multiple WD-40 repeats. The encoded protein mediates degradation of CD4 via its interaction with HIV-1 Vpu. It has also been shown to ubiquitinate phosphorylated NFKBIA (nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha), targeting it for degradation and thus activating nuclear factor kappa-B. Alternatively spliced transcript variants have been described. A related pseudogene exists in chromosome 6.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human β-TrCP/BTRC (NP_378663.1).
SequenceMDPAEAVLQEKALKFMCSMPRSLWLGCSSLADSMPSLRCLYNPGTGALTAFQNSSEREDCNNGEPPRKIIPEKNSLRQTYNSCARLCLNQETVCLASTAMKTENCVAKTKLANGTSSMIV
Gene ID
Swiss Prot
SynonymsFWD1; FBW1A; FBXW1; bTrCP; FBXW1A; bTrCP1; betaTrCP; BETA-TRCP; β-TrCP/BTRC
Calculated MW69kDa
Observed MW65kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesU-251MG
Cellular locationCytoplasm, Nucleus
Customer validation

WB(Mus musculus, Homo sapiens)

IF(Mus musculus)

Documents

Certificate of Compliance

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ABclonal:Western blot - β-TrCP/BTRC Rabbit pAb (A1656)}

Western blot - β-TrCP/BTRC Rabbit pAb (A1656)

Western blot analysis of lysates from U-251MG cells, using β-TrCP/BTRC Rabbit pAb (A1656) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
ABclonal:Immunohistochemistry - β-TrCP/BTRC Rabbit pAb (A1656)}

Immunohistochemistry - β-TrCP/BTRC Rabbit pAb (A1656)

Immunohistochemistry analysis of paraffin-embedded mouse spleen using β-TrCP/BTRC Rabbit pAb (A1656) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - β-TrCP/BTRC Rabbit pAb (A1656)}

Immunofluorescence - β-TrCP/BTRC Rabbit pAb (A1656)

Immunofluorescence analysis of C6 cells using β-TrCP/BTRC Rabbit pAb (A1656) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (1)

Secondary Antibodies (26)