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Tropomyosin 1 Rabbit pAb (A1157)

Review (1)Publications (5) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - Tropomyosin 1 Rabbit pAb (A1157)

Western blot analysis of various lysates using Tropomyosin 1 Rabbit pAb (A1157) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ABclonal:Immunofluorescence - Tropomyosin 1 Rabbit pAb (A1157)

Immunofluorescence analysis of HeLa cells using Tropomyosin 1 Rabbit pAb (A1157). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for Tropomyosin 1 Rabbit pAb(A1157)

Overview

Product nameTropomyosin 1 Rabbit pAb
Catalog No.A1157
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is a member of the tropomyosin family of highly conserved, widely distributed actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosin is composed of two alpha-helical chains arranged as a coiled-coil. It is polymerized end to end along the two grooves of actin filaments and provides stability to the filaments. The encoded protein is one type of alpha helical chain that forms the predominant tropomyosin of striated muscle, where it also functions in association with the troponin complex to regulate the calcium-dependent interaction of actin and myosin during muscle contraction. In smooth muscle and non-muscle cells, alternatively spliced transcript variants encoding a range of isoforms have been described. Mutations in this gene are associated with type 3 familial hypertrophic cardiomyopathy and dilated cardiomyopathy 1Y.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-245 of human Tropomyosin 1 (NP_001018008.1).
SequenceMAGSSSLEAVRRKIRSLQEQADAAEERAGTLQRELDHERKLRETAEADVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIESRAQKDEEKMEIQEIQLKEAKHIAEDADRKYEEVARKLVIIESDLERAEERAELSEGKCAELEEELKTVTNNLKSLEAQAEKYSQKEDRYEEEIKVLSDKLKEAETRAEFAERSVTKLEKSIDDLEDQLYQQLEQNRRLTNELKLALNED
Gene ID
Swiss Prot
SynonymsCMH3; TMSA; CMD1Y; LVNC9; C15orf13; HEL-S-265; HTM-alpha; Tropomyosin 1
Calculated MW33kDa
Observed MW36kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesA-549, BT-474, THP-1, DU145, SW480, MCF7, Mouse heart, Mouse skeletal muscle, Mouse brain
Cellular locationCytoplasm, cytoskeleton.
Customer validation

(Zebrafish)

WB(Danio rerio, Mus musculus, Homo sapiens, Other)

IF(Homo sapiens)

IHC(Mus musculus)

Documents

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ABclonal:Western blot - Tropomyosin 1 Rabbit pAb (A1157)}

Western blot - Tropomyosin 1 Rabbit pAb (A1157)

Western blot analysis of various lysates using Tropomyosin 1 Rabbit pAb (A1157) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunofluorescence - Tropomyosin 1 Rabbit pAb (A1157)}

Immunofluorescence - Tropomyosin 1 Rabbit pAb (A1157)

Immunofluorescence analysis of HeLa cells using Tropomyosin 1 Rabbit pAb (A1157). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (1)

Secondary Antibodies (26)