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γ-Tubulin Rabbit pAb (A6215)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - γ-Tubulin Rabbit pAb (A6215)

Western blot analysis of various lysates using γ-Tubulin Rabbit pAb (A6215) at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Western blot - γ-Tubulin Rabbit pAb (A6215)

Western blot analysis of various lysates using γ-Tubulin Rabbit pAb (A6215) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product nameγ-Tubulin Rabbit pAb
Catalog No.A6215
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the tubulin superfamily. The encoded protein localizes to the centrosome where it binds to microtubules as part of a complex referred to as the gamma-tubulin ring complex. The protein mediates microtubule nucleation and is required for microtubule formation and progression of the cell cycle. A pseudogene of this gene is found on chromosome 7.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 382-451 of human γ-Tubulin (NP_001061.2).
SequenceTSISSLFERTCRQYDKLRKREAFLEQFRKEDMFKDNFDEMDTSREIVQQLIDEYHAATRPDYISWGTQEQ
Gene ID
Swiss Prot
SynonymsTUBG; GCP-1; CDCBM4; TUBGCP1; γ-Tubulin
Calculated MW51kDa
Observed MW50kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesU2OS, HeLa, NIH/3T3, C2C12, Jurkat, C6, Mouse brain
Cellular locationCytoplasm, centrosome, cytoskeleton, microtubule organizing center.

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ABclonal:Western blot - γ-Tubulin Rabbit pAb (A6215)}

Western blot - γ-Tubulin Rabbit pAb (A6215)

Western blot analysis of various lysates using γ-Tubulin Rabbit pAb (A6215) at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - γ-Tubulin Rabbit pAb (A6215)}

Western blot - γ-Tubulin Rabbit pAb (A6215)

Western blot analysis of various lysates using γ-Tubulin Rabbit pAb (A6215) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

* For research use only. Not for therapeutic or diagnostic purposes.

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