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UAP56/DDX39B Rabbit pAb (A8356)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - UAP56/DDX39B Rabbit pAb (A8356)

Western blot analysis of various lysates using UAP56/DDX39B Rabbit pAb (A8356) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunohistochemistry - UAP56/DDX39B Rabbit pAb (A8356)

Immunohistochemistry analysis of paraffin-embedded Rat brain using UAP56/DDX39B Rabbit pAb (A8356) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - UAP56/DDX39B Rabbit pAb (A8356)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using UAP56/DDX39B Rabbit pAb (A8356) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - UAP56/DDX39B Rabbit pAb (A8356)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using UAP56/DDX39B Rabbit pAb (A8356) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - UAP56/DDX39B Rabbit pAb (A8356)

Immunofluorescence analysis of C6 cells using UAP56/DDX39B Rabbit pAb (A8356) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - UAP56/DDX39B Rabbit pAb (A8356)

Immunofluorescence analysis of NIH-3T3 cells using UAP56/DDX39B Rabbit pAb (A8356) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - UAP56/DDX39B Rabbit pAb (A8356)

Immunofluorescence analysis of U-2 OS cells using UAP56/DDX39B Rabbit pAb (A8356) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameUAP56/DDX39B Rabbit pAb
Catalog No.A8356
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the DEAD box family of RNA-dependent ATPases that mediate ATP hydrolysis during pre-mRNA splicing. The encoded protein is an essential splicing factor required for association of U2 small nuclear ribonucleoprotein with pre-mRNA, and it also plays an important role in mRNA export from the nucleus to the cytoplasm. This gene belongs to a cluster of genes localized in the vicinity of the genes encoding tumor necrosis factor alpha and tumor necrosis factor beta. These genes are all within the human major histocompatibility complex class III region. Mutations in this gene may be associated with rheumatoid arthritis. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on both chromosomes 6 and 11. Read-through transcription also occurs between this gene and the upstream ATP6V1G2 (ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G2) gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 319-428 of human UAP56/UAP56/DDX39B (NP_004631.1).
SequenceRGMPQEERLSRYQQFKDFQRRILVATNLFGRGMDIERVNIAFNYDMPEDSDTYLHRVARAGRFGTKGLAITFVSDENDAKILNDVQDRFEVNISELPDEIDISSYIEQTR
Gene ID
Swiss Prot
SynonymsBAT1; UAP56; D6S81E; UAP56/DDX39B
Calculated MW49kDa
Observed MW49kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:100 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples293T, HeLa, LO2, SKOV3, K-562, SW620
Cellular locationCytoplasm, Nucleus, Nucleus speckle

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - UAP56/DDX39B Rabbit pAb (A8356)}

    Western blot - UAP56/DDX39B Rabbit pAb (A8356)

    Western blot analysis of various lysates using UAP56/DDX39B Rabbit pAb (A8356) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 5s.
    ABclonal:Immunohistochemistry - UAP56/DDX39B Rabbit pAb (A8356)}

    Immunohistochemistry - UAP56/DDX39B Rabbit pAb (A8356)

    Immunohistochemistry analysis of paraffin-embedded Rat brain using UAP56/DDX39B Rabbit pAb (A8356) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - UAP56/DDX39B Rabbit pAb (A8356)}

    Immunohistochemistry - UAP56/DDX39B Rabbit pAb (A8356)

    Immunohistochemistry analysis of paraffin-embedded Human breast cancer using UAP56/DDX39B Rabbit pAb (A8356) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - UAP56/DDX39B Rabbit pAb (A8356)}

    Immunohistochemistry - UAP56/DDX39B Rabbit pAb (A8356)

    Immunohistochemistry analysis of paraffin-embedded Mouse brain using UAP56/DDX39B Rabbit pAb (A8356) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
    ABclonal:Immunofluorescence - UAP56/DDX39B Rabbit pAb (A8356)}

    Immunofluorescence - UAP56/DDX39B Rabbit pAb (A8356)

    Immunofluorescence analysis of C6 cells using UAP56/DDX39B Rabbit pAb (A8356) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - UAP56/DDX39B Rabbit pAb (A8356)}

    Immunofluorescence - UAP56/DDX39B Rabbit pAb (A8356)

    Immunofluorescence analysis of NIH-3T3 cells using UAP56/DDX39B Rabbit pAb (A8356) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - UAP56/DDX39B Rabbit pAb (A8356)}

    Immunofluorescence - UAP56/DDX39B Rabbit pAb (A8356)

    Immunofluorescence analysis of U-2 OS cells using UAP56/DDX39B Rabbit pAb (A8356) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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