제품 > 항체 > 다중클론항체(pAb)

UBN2 Rabbit pAb (A10516)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of various lysates using UBN2 Rabbit pAb (A10516) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product name	UBN2 Rabbit pAb
Catalog No.	A10516
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Predicted to be involved in DNA replication-independent chromatin assembly. Predicted to be located in nucleoplasm. Predicted to be active in nucleus. Is expressed in central nervous system and retina. Orthologous to human UBN2 (ubinuclein 2).

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 1-100 of mouse UBN2 (NP_796159.3).
Sequence	MAEPRRVAFISLSPVRRREADFAGAEREPPRLEPQPYREPARAEPAPRADAQPPARDKPLPQREVSRAEPPMALQREPPRPEPPPPPLPLQTPPPRESAS
Gene ID
Swiss Prot
Synonyms	mKIAA2030; 2900060J04Rik; 6030408G03Rik; D130059P03Rik; UBN2
Calculated MW	130kDa/131kDa/141kDa
Observed MW	190kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	K-562, 293T
Cellular location