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UCP3 Rabbit pAb (A1532)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - UCP3 Rabbit pAb (A1532)

Western blot analysis of various lysates using UCP3 Rabbit pAb (A1532) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Western blot - UCP3 Rabbit pAb (A1532)

Western blot analysis of various lysates using UCP3 Rabbit pAb (A1532) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - UCP3 Rabbit pAb (A1532)

Immunofluorescence analysis of C6 cells using UCP3 Rabbit pAb (A1532) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - UCP3 Rabbit pAb (A1532)

Immunofluorescence analysis of HeLa cells using UCP3 Rabbit pAb (A1532) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - UCP3 Rabbit pAb (A1532)

Immunofluorescence analysis of NIH/3T3 cells using UCP3 Rabbit pAb (A1532) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameUCP3 Rabbit pAb
Catalog No.A1532
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. The different UCPs have tissue-specific expression; this gene is primarily expressed in skeletal muscle. This gene's protein product is postulated to protect mitochondria against lipid-induced oxidative stress. Expression levels of this gene increase when fatty acid supplies to mitochondria exceed their oxidation capacity and the protein enables the export of fatty acids from mitochondria. UCPs contain the three solcar protein domains typically found in MACPs. Two splice variants have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human UCP3 (NP_073714.1).
SequenceMVGLKPSDVPPTMAVKFLGAGTAACFADLVTFPLDTAKVRLQIQGENQAVQTARLVQYRGVLGTILTMVRTEGPCSPYNGLVAGLQRQMSFASIRIGLYDSVKQVYTPKG
Gene ID
Swiss Prot
SynonymsSLC25A9; UCP3
Calculated MW34kDa
Observed MW34kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesSGC-7901, LO2
Cellular locationMitochondrion inner membrane, Multi-pass membrane protein.
Customer validation

WB(Sus scrofa, Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - UCP3 Rabbit pAb (A1532)}

Western blot - UCP3 Rabbit pAb (A1532)

Western blot analysis of various lysates using UCP3 Rabbit pAb (A1532) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - UCP3 Rabbit pAb (A1532)}

Western blot - UCP3 Rabbit pAb (A1532)

Western blot analysis of various lysates using UCP3 Rabbit pAb (A1532) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - UCP3 Rabbit pAb (A1532)}

Immunofluorescence - UCP3 Rabbit pAb (A1532)

Immunofluorescence analysis of C6 cells using UCP3 Rabbit pAb (A1532) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - UCP3 Rabbit pAb (A1532)}

Immunofluorescence - UCP3 Rabbit pAb (A1532)

Immunofluorescence analysis of HeLa cells using UCP3 Rabbit pAb (A1532) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - UCP3 Rabbit pAb (A1532)}

Immunofluorescence - UCP3 Rabbit pAb (A1532)

Immunofluorescence analysis of NIH/3T3 cells using UCP3 Rabbit pAb (A1532) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A1532? Please let us know so that we can cite the reference in this datasheet.

항체 (2)

Secondary Antibodies (26)