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UFC1 Rabbit mAb (A21154)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - UFC1 Rabbit mAb (A21154)

Western blot analysis of various lysates, using UFC1 Rabbit mAb (A21154) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 20s.

ABclonal:Western blot - UFC1 Rabbit mAb (A21154)

Western blot analysis of various lysates, using UFC1 Rabbit mAb (A21154) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.

Overview

Product nameUFC1 Rabbit mAb
Catalog No.A21154
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC3001
UFC1 is an E2-like conjugating enzyme for ubiquitin-fold modifier-1 (UFM1; MIM 610553) (Komatsu et al., 2004 [PubMed 15071506]).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-130 of Human UFC1 (NP_057490.2).
SequenceMADEATRRVVSEIPVLKTNAGPRDRELWVQRLKEEYQSLIRYVENNKNADNDWFRLESNKEGTRWFGKCWYIHDLLKYEFDIEFDIPITYPTTAPEIAVPELDGKTAKMYRGGKICLTDHFKPLWARNVP
Gene ID
Swiss Prot
SynonymsNEDSG; HSPC155; UFC1
Calculated MW19kDa
Observed MW20kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHepG2, MCF7, THP-1, Mouse pancreas, Rat pancreas
Cellular locationextracellular exosome

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - UFC1 Rabbit mAb (A21154)}

    Western blot - UFC1 Rabbit mAb (A21154)

    Western blot analysis of various lysates, using UFC1 Rabbit mAb (A21154) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 20s.
    ABclonal:Western blot - UFC1 Rabbit mAb (A21154)}

    Western blot - UFC1 Rabbit mAb (A21154)

    Western blot analysis of various lysates, using UFC1 Rabbit mAb (A21154) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.

    * For research use only. Not for therapeutic or diagnostic purposes.

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    Secondary Antibodies (26)