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UFC1 Rabbit pAb (A13192)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - UFC1 Rabbit pAb (A13192)

Western blot analysis of various lysates using UFC1 Rabbit pAb (A13192) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - UFC1 Rabbit pAb (A13192)

Immunofluorescence analysis of C6 cells using UFC1 Rabbit pAb (A13192) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - UFC1 Rabbit pAb (A13192)

Immunofluorescence analysis of NIH-3T3 cells using UFC1 Rabbit pAb (A13192) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - UFC1 Rabbit pAb (A13192)

Immunofluorescence analysis of U-2 OS cells using UFC1 Rabbit pAb (A13192) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameUFC1 Rabbit pAb
Catalog No.A13192
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
UFC1 is an E2-like conjugating enzyme for ubiquitin-fold modifier-1 (UFM1; MIM 610553) (Komatsu et al., 2004 [PubMed 15071506]).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-167 of human UFC1 (NP_057490.2).
SequenceMADEATRRVVSEIPVLKTNAGPRDRELWVQRLKEEYQSLIRYVENNKNADNDWFRLESNKEGTRWFGKCWYIHDLLKYEFDIEFDIPITYPTTAPEIAVPELDGKTAKMYRGGKICLTDHFKPLWARNVPKFGLAHLMALGLGPWLAVEIPDLIQKGVIQHKEKCNQ
Gene ID
Swiss Prot
SynonymsNEDSG; HSPC155; UFC1
Calculated MW19kDa
Observed MW19kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesU-87MG, A-549, Mouse brain, Mouse kidney
Cellular locationextracellular exosome

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - UFC1 Rabbit pAb (A13192)}

    Western blot - UFC1 Rabbit pAb (A13192)

    Western blot analysis of various lysates using UFC1 Rabbit pAb (A13192) at 1:3000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Immunofluorescence - UFC1 Rabbit pAb (A13192)}

    Immunofluorescence - UFC1 Rabbit pAb (A13192)

    Immunofluorescence analysis of C6 cells using UFC1 Rabbit pAb (A13192) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - UFC1 Rabbit pAb (A13192)}

    Immunofluorescence - UFC1 Rabbit pAb (A13192)

    Immunofluorescence analysis of NIH-3T3 cells using UFC1 Rabbit pAb (A13192) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - UFC1 Rabbit pAb (A13192)}

    Immunofluorescence - UFC1 Rabbit pAb (A13192)

    Immunofluorescence analysis of U-2 OS cells using UFC1 Rabbit pAb (A13192) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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