제품 > 항체 > 다중클론항체(pAb)

USP42 Rabbit pAb (A15911)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - USP42 Rabbit pAb (A15911)

Western blot analysis of various lysates using USP42 Rabbit pAb (A15911) at 1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 160s.

Overview

Product nameUSP42 Rabbit pAb
Catalog No.A15911
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables thiol-dependent deubiquitinase. Involved in protein deubiquitination. Predicted to be located in nucleoplasm. Predicted to be active in cytosol and nucleus.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 500-700 of human USP42 (NP_115548.1).
SequenceASVQNWSVNRSSVIPEHPKKQKITISIHNKLPVRQCQSQPNLHSNSLENPTKPVPSSTITNSAVQSTSNASTMSVSSKVTKPIPRSESCSQPVMNGKSKLNSSVLVPYGAESSEDSDEESKGLGKENGIGTIVSSHSPGQDAEDEEATPHELQEPMTLNGANSADSDSDPKENGLAPDGASCQGQPALHSENPFAKANGLP
Gene ID
Swiss Prot
SynonymsUSP42
Calculated MW145kDa
Observed MW170kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHT-29, Raji, SGC7901, Mouse testis
Cellular locationcytosol, nucleoplasm, nucleus
    ABclonal:Western blot - USP42 Rabbit pAb (A15911)}

    Western blot - USP42 Rabbit pAb (A15911)

    Western blot analysis of various lysates using USP42 Rabbit pAb (A15911) at 1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 160s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)